ANTIFUNGAL AND SYNERGISTIC EFFECTS OF ZNO NANOPARTICLES AGAINST T.VERRUCOSUM CAUSED RINGWORM IN COWS
DOI:
https://doi.org/10.36103/ijas.v54i3.1770Keywords:
Trichophyton, synergism treatment,Abstract
The current study was aimed to determined the main causes of ringworm in cows and antifungal and synergistic effects of ZnO nanoparticles. For this purpose 50 skin scrapes were collected from cows infected with ringworm, culture media, staining and genetic methods used for diagnosis. MIC and MFC for antifungal and ZnO were determined. The result showed that Trichophyton spp was isolated in rate of 76%. The isolation rate of T.verrucosum, T. mentagrophytes and T. rubrum were 68.4%, 21.0% and 10.5% respectively. MIC of Nystatin, fluocytosin, ZnO, Nystatin+ ZnO and Fluocytosin + ZnO were 200,150,200,150 and 100 μg/ml respectively. in conclusion, that T.verrucosum is main caused of Ringworm and ZnO has antifungal and synergistic effects.
References
Abbas, L. M. R., A. J Hashim and W. Al-Hadban, 2020. Identification local isolates of Trichophyton mentagrophytes and detection of keratinase gene using pcr technique. Iraqi Journal Of Agricultural Science, 51(6): 1534-1542. https://doi.org/10.36103/ijas.v51i6.1181
Agarwal, H., S. Menon, S. V Kumar and S. Rajeshkumar, 2018. Mechanistic study on antibacterial action of zinc oxide nanoparticles synthesized using green route. Chemico-biological Interactions, 286: 60-70
Agbulu, C. O., C.Iwodi, and A. Onekutu, 2015. In vitro susceptibility test of some antifungal drugs on selected Dermatophytes and yeasts isolated from patients attending hospitals in Makurdi environ. Microbiol. J., 5: 9-16
Agnetti, F., R. Ciavarella, D. Cruciani, E. M. Epifanio, D. Golinelli, P. Papa and S. Crotti, 2020. Ringworm by Trichophyton erinacei in calves: description of two Italian outbreaks. Large Animal Review, 26(3): 141-143
Álvarez, N. H., L. N. Fernandez, G. M. Seimandi, M. I. Stegmayer, V. E. Ruiz and M. G. Derita, 2021. Antifungal Activity Validation of Wild Plants used in Argentine Ethnomedicine: A Revision of Polygonum. In Ethnopharmacology of Wild Plants (pp:111-128). CRC Press
Cabanes, F. J. 2020. Revista Iberoamericana de Micología. Rev Iberoam Micol, 37(1).
Chand, P., S. Kumari, N. Mondal, , S. P. Singh and T. Prasad 2021. Synergism of Zinc Oxide Quantum Dots with Antifungal Drugs: Potential Approach for Combination Therapy against Drug Resistant Candida albicans. Frontiers in Nanotechnology, 3: 32
Chowdappa, A., S. Patil, and M. B. Nagmoti, 2020. Isolation and Molecular Characterization of Trichophyton rubrum from Siddi Tribal Community Residing in North Karnataka Region, India. Journal of Evolution of Medical and Dental Sciences, 9(31):2208-2212
Fatima, D. 2021. Fungal Diseases of Bovines. In Fungal Diseases in Animals (pp: 1-14): Springer, Cham
Geethalakshmi, V., K. Jasmine, A. P. John and P. Prathap, 2021. Effectiveness Of sabouraud's dextrose agar and dermatophyte test medium in detection of candidiasis and dermatophytosis in superficial skin lesion. Journal Of Clinical & Diagnostic Research, 15(8).
Giordo, R., G. K. Nasrallah, O. Al-Jamal, P. Paliogiannis and G.Pintus, 2020. Resveratrol inhibits oxidative stress and prevents mitochondrial damage induced by zinc oxide nanoparticles in zebrafish (Danio rerio). International journal of molecular sciences, 21(11): 3838
González‐Merino, A. M., A. Hernández‐Juárez, R. Betancourt‐Galindo, Y. M. Ochoa‐Fuentes, L. A. Valdez‐Aguilar and M. L. Limón‐Corona, 2021. Antifungal activity of zinc oxide nanoparticles in Fusarium oxysporum ‐ Solanum lycopersicum pathosystem under controlled conditions. Journal of Phytopathology, 169(9): 533-544
Guo, Y., S. Ge, H. Luo, A. Rehman, Y. Li and S. He 2020. Occurrence of Trichophyton verrucosum in cattle in the Ningxia Hui autonomous region, China. BMC Veterinary Research: 16(1): 1-9
Hasso, S. A. 2016. An update review of confirmed pathogens of six animal species in Iraq. Iraqi Journal of Veterinary Sciences: 30(1): 15-17
Hayette, M. P., and Sacheli, R. 2021. Unusual Dermatophytosis Presentations and New Emerging Dermatophytes Species. In Dermatophytes and Dermatophytoses (pp: 87-114). Springer, Cham
Jong, S. C., and F. M. Dugan, 2002. The Order Entomophthorales. Pathogenic Fungi in Humans and Animals, 127.
K Zangana, I., and A. N.Issa, 2009. Isolation of Trichophyton mentogrophytes var mentogrophytes from naturally infected laboratory albino rats: Experimental infection and treatment in rabbits. Iraqi Journal of Veterinary Sciences, 23(2): 29-34
Li, T., L. Li, F. Du, L. Sun, J. Shi, M. Long, and Z.Chen, 2021. Activity and mechanism of action of antifungal peptides from microorganisms:A Review. Molecules, 26(11), 3438
Lindenhahn, J., T.Bartosch, C. M. Baumbach, M. Suchowski, J. Kacza, W. Schrödl and J. K. Michler, 2021. Detection of subtilisin 3 and 6 in skin biopsies of cattle with clinically manifested bovine ringworm. Medical Mycology, 59(3): 305-308
Mishra, V., M. Singh, Y. Mishra, N. Charbe, P.Nayak, K. Sudhakar, and M. M. Tambuwala, 2021. Nanoarchitectures in Management of Fungal Diseases: An Overview. Applied Sciences, 11(15): 7119
Mohamed, A. A., M. Abu-Elghait, , N. E Ahmed, and S. S. Salem, 2021. Eco-friendly mycogenic synthesis of ZnO and CuO nanoparticles for in vitro antibacterial, antibiofilm, and antifungal applications. Biological Trace Element Research, 199(7): 2788-2799
Mohammed, S. J. 2011. A survey of dermatophytes isolated from cows and sheep in Iraq. The Iraqi Journal of Veterinary Medicine, 35(2): 40-45
Nwofor, C. N., C. A. Oyeka, N. E. Onyenwe, M. O. Echeta, and Y. M.Tatfeng, 2021. Prevalence of Non-Dermatophytic Molds Associated with Cutaneous Mycoses in Cattle in Abia and Imo States, Nigeria. Archives of Current Research International,pp: 43-56.
Pillai, A. M., V. S. Sivasankarapillai, A. Rahdar, J. Joseph, F. Sadeghfar, K. Rajesh, and G. Z. Kyzas 2020. Green synthesis and characterization of zinc oxide nanoparticles with antibacterial and antifungal activity. Journal of Molecular Structure, 1211: 128107
Salari, S., S. A. A. Mousavi, S. Hadizadeh and A. Izadi, 2017. Epidemiology of dermatomycoses in Kerman province, southeast of Iran: a 10-years retrospective study (2004–2014). Microbial pathogenesis, 110: 561-567
Sardar, M., W. Ahmed, S. Al Ayoubi, S. Nisa, Y. Bibi, M. Sabir and A. Qayyum, 2021. Fungicidal synergistic effect of biogenically synthesized zinc oxide and copper oxide nanoparticles against Alternaria citri causing citrus black rot disease. Saudi Journal of Biological Sciences. 31(4), 105-108
Jamil, A., N. Musheer and S. Ashraf, 2021. Antagonistic potential of Trichoderma harzianum and Azadirachta indica against Fusarium oxysporum f. sp. capsici for the management of chilli wilt. Journal of Plant Diseases and Protection, 128(1): 161-172
Shourie, A., R. Singh, and A. Singh, 2021. Histopathologic Diagnosis of Fungal Infections of Lab Animals. In Fungal Diseases in Animals (pp: 73-91). Springer, Cham
Sruthi, S., J. Ashtami and P. V.Mohanan, 2018. Biomedical application and hidden toxicity of Zinc oxide nanoparticles. Materials Today Chemistry, 10: 175-186
Stegmayer, M. I., V. E. Ruiz, and M. G Derita,. 2021. Antifungal Activity Validation of Wild Plants used in Argentine Ethnomedicine. Ethnopharmacology of Wild Plants, 111
Yang, D., M. Zhang, Y. Gan, S. Yang, J. Wang, M. Yu, and J. Chen, 2020. Involvement of oxidative stress in ZnO NPs-induced apoptosis and autophagy of mouse GC-1 spg cells. Ecotoxicology and Environmental Safety, 202: 110960
Yassin, S. G., and B. T. Mohammed,. 2021. Evaluation of mineral, nano-zinc and fluconazole interaction on some growth characteristics of Trichophyton rubrum and Microsporum canis. Biochem. Cell. Arch, 21(1): 1359-1369
Zhu, J., S. Liu, Y. Qin, R. E. Xing, H. Yu, X. Chen and P. Li, 2020. Preparation, characterization, and antifungal evaluation of a new type of aminourea chitooligosaccharide derivatives. Journal of Oceanology and Limnology, 38(3):841-850.
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