THE PREVALENCE OF INTEGRON CLASS I AND II AMONG MULTI-DRUG RESISTANCE PRODUCING KLEBSIELLA pneumonia
DOI:
https://doi.org/10.36103/ijas.v51i5.1739Keywords:
Bacteria, Antibiotics, MolecularAbstract
This study aimed to determine antibiotic resistance profiles and the distribution of Integron I, Integron II, and genes among uropathogenic Klebsiella pneumoniae isolated from patients suffering from urinary tract infection. The results revealed that one hundred and thirty-seven (91%) out of 150 clinical specimens were Klebsiella pneumoniae. The isolates (137 isolates) were given a higher percentage of resistance against Piperacillin (94.89%)and Rifampicin (93.43%) and the lowest resistance was with Meropenem (40.87%). According to PCR amplification for 15 isolates showed the proportion of class I (intl1) and class II (intl2) integrons were investigated. What are 100% (n=15) and 45% (n=7) of isolates had intl1 and intl2 genes, respectively. PCR products for (Five)isolates were subjected to direct sequencing, and both strands of PCR products were sequenced with an automatic sequencer. These sequences were analyzed for the presence of variance of these genes and the detection of the differences in the nucleotides.
References
Ahmed Hasan, S., T. Fakhraddin Raheem, and H.Mohammed Abdulla, 2021. Phenotypic, Antibiotyping, and Molecular Detection of Klebsiella Pneumoniae Isolate from Clinical Specimens in Kirkuk, Iraq. Archives of Razi Institute, 76(4), 1061-1067.
Asghari, B., R. Goodarzi, M. Mohammadi, F. Nouri, and M. Taheri, 2021. Detection of mobile genetic elements in multidrug-resistant Klebsiella pneumoniae isolated from different infection sites in Hamadan, West of Iran. BMC Research notes, 14(1), 330.
Biksi, I. 2002. Some aspects of urogenital tract diseases of female breeding swine. Hungarian Vet Arc. HTTP:// HDL. handle. net/10832/556
Budiarso, T. Y., C. Amarantini, and S. Pakpahan, 2021. Biochemical identification and molecular characterization of Klebsiella Pneumoniae isolated from street foods and drinks in Yogyakarta, Indonesia using 16S rRNA gene. Biodiversitas Journal of Biological Diversity, 22(12). 5452-5458
Calfee D. P. 2017. Recent advances in the understanding and management of Klebsiella pneumoniae. F1000Research, 6, 1760.
Cronan, M. R., E. J. Hughes, W. J.Brewer, G. Viswanathan, E. G. Hunt, B. Singh, S. H. Mehra, S. G. Oehlers, S. Gregory, D. Kaushal, and D. M. Tobin, 2021. A non-canonical type 2 immune response coordinates tuberculous granuloma formation and epithelialization. Cell, 184(7), 1757 1774.e14
Delarampour, A., Z. R. Ghalehnoo, F. Khademi, and H. Vaez, 2020. Antibiotic resistance patterns and prevalence of class I, II, and III Integrons among clinical isolates of Klebsiella pneumoniae. Le Infezioni in Medicina, 28(1): 64-69.
Deng, Y., X. Bao, L. Ji, L. Chen, J. Liu, J. Miao, D. Chen, H. Bian, Y., Li, and G. Yu, 2015. Resistance integrons: class 1, 2 and 3 integrons. Annals of Clinical Microbiology and Antimicrobials, 14, 45.
Domingues, S., G. J. da Silva, and K. M. Nielsen, 2015. Global dissemination patterns of common gene cassette arrays in class 1 integrons. Microbiology (Reading, England), 161(7), 1313-1337
Fatihu,M.Y.and P.B. Addo, 1991. Isolation of Corynebacterium renalefrom slaughtered cattle at the Zaria abattoir in Nigeria. Rev d’él et de méd vét des pay tro.44(2):160-161
Firoozeh, F., Z. Mahluji, A. Khorshidi, and M. Zibaei, 2019. Molecular characterization of class 1, 2, and 3 integrons in clinical multi-drug resistant Klebsiella pneumoniae isolates. Antimicrobial Resistance and Infection Control, 8, 59
Flores-Mireles, A. L., J. N. Walker, M. Caparon, and S. J. Hultgren, 2015. Urinary tract infections: epidemiology, mechanisms of infection and treatment options. Nature reviews. Microbiology, 13(5), 269-284.
Heidary, M., M.J. Nasiri, H. Dabiri, and S. Tarashi, 2018. Prevalence of Drug-resistant Klebsiella pneumoniae in Iran: A Review Article. Iranian Journal of Public Health, 47(3), 317-326.
Ikram, R., R. Psutka, A. Carter, and P. Priest, 2015. An outbreak of multi-drug resistant Escherichia coli urinary tract infection in an elderly population: a case-control study of risk factors. BMC Infectious Diseases, 15, (224):,
Jahanbin, F., M. Marashifard, S. Jamshidi, M. Zamanzadeh, M. Dehshiri, S. Malek Hosseini, and S. S. Khoramrooz, 2020. Investigation of Integron-Associated Resistance Gene Cassettes in Urinary Isolates of Klebsiella pneumoniae in Yasuj, Southwestern Iran During 2015-16. Avicenna Journal of Medical Biotechnology, 12(2): 124-131.
Kirby,W.M. andA.W. Bauer. 1966. Antibiotic susceptibility testing by a standardized single disc method. Ame J Clin Path.45:493-496. doi.org/10.1093/ajcp/45.4_ts.493
Li, B., Y. Hu, Q. Wang, Y. Yi, P. C. Woo, H. Jing, B. Zhu, and C. H. Liu, 2013. Structural diversity of class 1 integrons and their associated gene cassettes in Klebsiella
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
How to Cite
2.jpg)
https://orcid.org/0000-0002-5774-5906 2.jpg)
