PREVALENCE OF ENTEROTOXIN TYPE A IN STAPHYLOCOCCUS AUREUS ISOLATED FROM DIFFERENT SOURCES

Authors

  • B. A. Kadhim
  • M. A. Essa
  • M. R. Sabbah

DOI:

https://doi.org/10.36103/ijas.v53i6.1648

Keywords:

drinking water, ELISA, optical density, food, soil .

Abstract

This research was aimed to investigate the prevalence of enterotoxin type A in (82) Staphylococcus aureus isolates from different sources(Pathogenic, Soil, Food, Tape water& Nasal swabs from Normal source), the results had shown the capacity of (58) isolates (70.73%) to produce the enterotoxin by using culture method (in solid and liquid medium), concerning the liquid medium, the results conducted that there was a significant differences at P£ 0.05,  among bacterial isolates in the amount of produced enterotoxin in terms of optical density. By using serological ELISA  specific kit, the results had confirmed the ability of (47) bacterial isolates(57.32%)  to produce enterotoxin type A with concentrations were ranged between( 3.7 - 15.7) ng/ml, which represents (81%) of all isolates producing enterotoxins, also the results indicated that there were isolates from different isolation sources can produce this toxin , and the prevalence of enterotoxin type A producing isolates was in pathogenic isolates(43.9%), soil (66.7%), food(92.3%), Tape water(55%) & Nasal swabs(80%), This prevalence of enterotoxin type A which is the most dangerous of Staphylococcal enterotoxins in studied  isolates reflects the risk and healthy importance of this bacteria, regardless of the source of its isolation.

References

Abril, A. G.; T. G., Villa; J. B. Velazquez; B., Canas, A. S., Perez; P. C. Mata and M. Carrera .2020. Staphylococcus aureus exotoxins and their detection in the dairy industry and mastitis .Toxin. 12:537- 554..

Balaban, N. and A. Rassoly . 2000. Staphylococcal enterotoxins. Int. J. Food. Microbiol.61(1-10).

Boynukara, P.B.; T. Gulhan; M.A.Sarli; K. Gurturk, and H ., Solmaz. 2008. Classical enterotoxigenic characteristics of S.aureus strain isolated from bovine subclinical Mastitis in Van, Turkey .Int.J.Food. Microbiol. 125(2):209-211

Carfora, V.; A.Caprioli,; N.Marri; D.Sagrafoli ; C., Boselli and G., Giacinti. 2015. Enterotoxin, genes, enterotoxin production and methicillin resistance in S.aureus isolated from milk and dairy products in central Italy. Int. Dairy J. 42:12-15

Cencigoga, B.T.; M. Karama; P.V., Rossitto ; R.A., Morgante and J.S., Cullor. 2003. Eenterotoxin production by S.aureus Isolated from mastitis cow. J.Food. Prot. 66(9): 1693-1696.

Cheng, M. H.; S., Zhang; R. A, Porritt; M. V., Rivas; L. Paschold; E. Willescher,; M., Binder; M. Arditi, and I., Bahar. 2020. Superantiginic character of an insert unique to SARS-COV-2 spike supported by skewed TCR repertoire in patients with hyperinflamation .PNAS.117(41):25254-25262

Clinical and Laboratory Standards Institute. 2010. Methods for antimicrobial dilution and disk susceptibility testing of infrequeutly isolatated or fastidious bacteria ; Approved guideline, 2nd. ed. M45-A2. CLSI, Wayne, PA.pp:78-82

Cowan, M. K. and K. P., Talaro. 2006. Micorbiology A System Approach, 1st. ed. McGraw Hill Company. pp : 21.

Daracova, M.; J., Klimentova; A.Fucikova and J., Dresler. 2018 . Proteomic methods of detection and quantification of protein toxin .Toxin. 10:99-128

Doyle, M. E.; F. A., Hartmann and A. C., Lee Wong. 2012. Methicillin- resistant Staphylococci: Implications for our food supply . Anim. Health Res. Rev.,13(2):157-180

Fisher,E.L.; M., Otto. and Y.C., Cheung. 2018. Basis of virulence in enterotoxin mediated staphylococcal food poisoning. Front. Microbiol. 9:436(1-18).

General Organization for Technical Education and Vocational Training .2009. Spectroscopy Methods. KSA . pp:16.

Hait, T.; S .,Talent; D.,Melka; C., Keys and R. , Bennett. 2014. Prevalence of enterotoxins and toxin gene profile of S. aureus isolates recovers from a bakery involved in a second staphylococcal food poisoning occurrence. J.Appl. Mccrobiol.117:866-875.

Hassan, K.I and S.K. ,Hamasaleh .2016. A rapid methods for PCR based on detection of Salmonella spp. and S. aureus in Spiked and naturally contaminated food . Iraqi J. Agric . Sci. 47(7): 66-73

Hen, M. M. S.; W. S. J., Boardman; I., Smith; A. E., Goodman and M. H.,Brown. 2014. Nasal colonization of Staphylococcus spp. among captive and free ranging Wallabies in South Australia . J. Vet. Sci. Med. Diag.. 3(2):136- 144.

Ibrahim, R. N.; M. S., Al- Salmani and T.H. Zedan. 2019. Study the antibacterial activity of aqueous extraction of onion (Allium cepalae L.) against Staphylococuus aureus isolated from otitis media. Iraqi J. Agric. Sci. 50(4): 1186-1192.

Jassim, S. A. and N. J. Kandala. 2021. Molecular detection of enterotoxin genes of multi resistant Staphylococcus aureus isolated from different source of food. Iraqi Journal of Science .62(1): 61-74

Johler, S.; P., Giannini; M., Jemini ; J., Hummerjohann ; A. , Baumgartner and R. Stephan. 2015. Further evidence for Staphylococcal food poisoning out breaks Caused by egc- encoded enterotoxins. Toxins, 7:997-1004

Joo, H. S.; S. S., Chatterjee; A. E., Villaruz; S. W., Dickey; V. Y., Tan; Y., Chen; D. E., Sturdevant ; S. M. ,Ricklefs and M., Otto. 2016. Mechanism of gene regulation by a Staphylococcus aureus toxin .Ameri. Socie. Microbiol.J.7(5):16-25

Jusko, M.; T., Potempa; T., Kantyka; E., Bielecka; H.K., Miller; M., Kalinska; G., Dubin; P., Garred; L.N. ,Shaw and A.M. , Blom, 2014. Staphylococcal proteases aid in evasion of the human complement system. J. Innate Immun., 6(1):31-46.

Liu, J.; L.W., Bai ; H., Han; P., Fu; X., Ma; Z., Bi; X.,Yong ; X. , Zhang and S.,Zhen . 2018. Trends of food borne disease in China: Lessons from laboratory based surveillance since 2011. Front. Med.12:48-57

Mohammed, S.A.; M.I.J., Al- Ani; D.L., Mohammed ; L.R. , Salar and B.M., Rasul. 2019. Inhibition of S. aureus enterotoxin genes by using plant extracts. Innovaciencia.6(2): 1-8.

Nadhom, B.N. 2018 . Study of molecular composition of Virulence bacteria isolated from bovine mastitis with biofilm production. Iraqi J. Agric . Sci. 49(5): 840-846.

Norri, H.A and G.M., Aziz. 2020. Purification, Characterization, and evaluation of fibrinolytic activity of staphylokinase from locally isolated Staphylococcus aureus GH38. Iraq.J.Agric.Sci. 51(4):1195-1203

Omoe, K.; D.L., Hu; H.K., One ; S., Shimizu; O.H.,Takahashi ; A.,Nakane ; T., Uchiyama ; K. , Shinagawa, and K., Imanishi. 2013. Emetic potentials of newly staphylococcal enterotoxin like toxins. Infect. Immun. 81:3627-3631

SAS.2012.Sraristical Analysis System, User's Guide.Statistical. version. 9.1th ed.SAS. Inst.Inc.Cary.N.C.USA.pp:23-25.

Starr, C.; C.A. , Evers and L ., Starr. 2006. Biology Concepts and Applications. Thomson, Books 1 cole, Brojstranica.pp :54

Viennot, L. and C.D. , Hosson. 2012. Colour Phenomena and partial Absorption. MUSE group .European Physical Society. pp :6

Zhao, Y.; A., Zhu; C.,Tang ; J., Chen and J., Liu. 2016. Identification and measurement of staphylococcal enterotoxin like protein I(SE/I) secretion from Staphylococcus aureus clinical isolates. J. Appl.Microbiol.121:539-546.

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2022-12-29

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How to Cite

Kadhim, B. A., Essa , M. A., & Sabbah, M. R. (2022). PREVALENCE OF ENTEROTOXIN TYPE A IN STAPHYLOCOCCUS AUREUS ISOLATED FROM DIFFERENT SOURCES. IRAQI JOURNAL OF AGRICULTURAL SCIENCES, 53(6), 1325-1330. https://doi.org/10.36103/ijas.v53i6.1648

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