SECONDARY COMPOUNDS RELEASED BY RHIZOSPHERIC BACTERIA EXHIBIT FUNGISTATIC EFFECTS AGAINST PHYTOPATHOGENIC FUNGUS.
Keywords:biocontrol, rhizospheric bacteria, fungistatic activity, volatile organic compounds.
The aim of conducted research was to devalute the inhibition effect of metabolites secreted by some Rhizospheric bacteria on the growth of two pathogenic fungi: Rhizoctonia solani and Fussarium solani, 330 bacteria isolates were obtained from the rhizosphere of the wheat, tomato, cowpea and Mt. Atlas mastic tree grown in the Sulaimani. Thirty-five isolates were screened for suppression of phytopathogeneic fungus. Results showed that most of these rhizospheric bacteria were have fungistatic potential in different degrees. The highest inhibition of the linear growth of fungi was noted for R. solani and F. solani, by Bacillus cereus LXJ73 (90.41%), Bacillus atropheaus SM-1 (75%) respectively. Six bacterial isolates were selected which have potential effect on the growth of the two studied fungi, five isolates belonging to the Bacillus genus and one isolate belonging to the genus Klebsiella. these isolates were molecularly confirmed by 16SrRNA, and their activity for inhibiting fungal growth were studied. The extracted metabolities analysied by Gas chromatography-Mass spectrometry (GC-MS), showing that extracts contain different volatile organic compounds as Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-, 2-Pentanone, 4-hydroxy-4-methyl-, Isosteviol methyl ester,2-methyl-, Butanoic acid, 3-methyl-, 3-Pyrrolidin-2-yl-propionic acid, 9-Octadecenamide, (Z)-, Hexadecane, Tridecane, 2,5-Piperazinedione, 3,6-bis(2-methyl propyl)-, 1,2-Benzenedicarboxylic acid, diisooctyl ester, Acetic acid, Dodecane, beta.-D-Glucopyranose, 1,6-anhydro-, Lauric acid, 2-(hexadecyloxy)-3-(octadecyloxy)propyl ester and cis-Vaccenic acid. The presence of these componuds indicate that all studied Bacillus species and Klebsiella michiganensis M1-3-11having antagonistic activity and they can be used for development of biocontrol agents to reducing the phytopathogenic problems and decreasing mycotoxine in contaminated crops.
Ajilogba, Caroline F., and O. Olubukola Babalola. 2019. GC–MS Analysis of volatile organic compounds from bam –bara groundnut rhizobacteria and their antibact- erial properties.World Journal of Micro biology and Biotechno logy 35(6):1–19.https://doi.org/10.1007/s112 74-019-26607
Anelise Beneduzi, Adriana Ambrosini, and Luciane M.P. Passaglia. 2012. Plant growth-promoting rhizobacteria (PG PR)_ their potential as antagonists and biocontrol agents. https://doi.org/10.15 90/s1415- 47572012000600020
Aydi Ben Abdallah, Rania, Hayfa Jab noun-Khiareddine, Ahlem Nefzi, Sonia Mokni-Tlili, and Mejda Daami-Remadi. 2016. Biocontrol of fusarium wilt and growth promotion of tomato plants using endophytic bacteria isolated from Sola num elaeagnifolium Stems. Journal of Phyto pathology1 64(10).https: //doi.or g/10.1111/j ph.12501
Backer, Rachel, J. Stefan Rokem, Gayathri Ilangumaran, John Lamont, Dana Praslickova, Emily Ricci, Sowmyalakshmi Subramanian, and Donald L. Smith. 2018 . Plant growth-promoting rhizobacteria: context, mechanisms of action, androad map to commercialization of biostimulants for sustainable agriculture. Fron tiers in Plant Science. Frontiers Media S.A. https://doi.org /10.3389/fpls.2018.01473.
Bharose, A.A, and H. P. Gajera. 2018. Antifungal activity and metabolites study of Bacillus strain against aflatoxin producing Aspergillus. Journal of Applied Microbiology and Biochemistry 02(02):1–8.https://doi.org/10.21767/257 6-1412.100 024]
Cawoy, Hélène, Delphine Debois, Laurent Franzil, Edwin De Pauw, Philippe Thonart, and Marc Ongena. 2015. Lipo peptides as main ingredients for inhibition of fungal phytopathogens by Bacillus subtilis /amyloliquefaciens. Microbial Biotechnology 8 (2): 281–95. https: //doi.org /10.1111 /1751-7915 .12 238.
Egamberdieva, Dilfuza, Stephan Wirth, Undine Behrendt, Parvaiz Ahmad, and Gabriele Berg. 2017. Antimicrobial activity of Medicinal Plants Correlates with the proportion of antagonistic endophytes. Frontiers in Microbiology 8 (February).https://doi.Org/10.3389/fmic b. 2017.00 199.
Guevara-Avendaño, Edgar, Alix Adr iana Bejarano-Bolívar, Ana Luisa Kiel-Martínez, Mónica Ramírez-Vázquez, Alfo n so Méndez-Bravo, Eneas Aguirre von Wobeser, Diana Sánchez-Rangel, José A. Guerrero-Ana lco,Akif Eskalen, and Frédérique Reverchon. 2019. Avocado rhizobacteria emit volatile organic comp ounds with antifungal activity against Fusarium solani, Fusarium Sp. associated with kuroshio shot hole borer, and colletotrichum gloeos porioides. Microbiological Resea- rch 219:74–83. https://doi.org/10.101 6/ j.micres .2018.11.009
Guevara-Avendaño, Edgar, Joseph D. Carrillo, Cedric Ndinga-Muniania, Kevin Moreno, Alfonso Méndez-Bravo, José A. Guerrero-Analco, Akif Eskalen, and Frédérique Reverchon. 2018. Antifungal activity of avocado rhizo bacteria against Fusarium euwa llaceae and graphium Spp., associated with euwallacea Spp. Nr. fornicatus, and phytophthora cinna momi. Antonievan Leeuwenhoek, International Journal of General and Molecular Micro -biology 111 (4):563–72. https://doi.org/10.1007 / s10482-017-0977-5
He, Chao Nan, Wan Qiong Ye, Ying Ying Zhu, and Wen Wen Zhou. 2020. Antifungal activity of volatile organic compounds produced by Bacillus methyl otrophicus and Bacillus thuringiensis against five common spoilage fungi on Loquats. Molecules 25(15).https://doi. org/ 10.3390/molecules 25153360
Hynes, Russell K., Grant C.Y. Leung, Danielle L.M. Hirkala, and Louise M. Nelson. 2008. Isolation, selection, and characterization of beneficial rhizobact- eria from pea, lentil, and chickpea grown in western canada. Canadian Journal of Microbiology 54 (4): 248–58.https:// doi.org /10. 1139 /W 08-008
Jebur, H.A.and Auda, J.M. 2020. Eval ution of antimicrobial activity of partialpuri fied bacteriocin from local isolate of Bacill us licheniforims HJ2020 MT192715.1. Iraqi Journal of Agricul tur al Sciences – 51 (6): 1644–52
Liu, Xiang, Shengman Lyu, Shurong Zhou, and J.A. Corey Bradshaw. 2016. Warming and fertilization alter the dilution effect of host diversity on disease severity. Ecology. https://doi org/10.1890/ 15-1784.1
Manal, M. El-Naggar, A. H. Ibrahim Hassan, G. Battah Mohamed, Kh Abd Al-Gawad Fagr,and S. Ibrahim Mohamed.2014. Antifungal agent prod -uction from a new marine Bacillus pumilus SMH101. African Journal of Microbiology Research 8 (3): 286–96. https://doi.org/10.5897/ajmr2013. 6379
Meena, K. R., and S. S. Kanwar, 2015. Lipopeptides as the antifungal and anti bacterial agents: applications in food safety and therapeutics.BioMed research international, 473050. https://doi. org/10. 1155/2015/473050
Miles, A A, S S Misra, and J 0 Irwin. 2021. The estimation of the bactericidal power of the blood with a note. https:// doi.org/10.1017/S002217240001158X
Mnif, Ines, Ines Hammami, Mohamed Ali Triki, Manel Cheffi Azabou, Semia Ellouze-Chaabouni, and Dhouha Ghribi. 2015. Antifungal efficiency of a lipo peptide biosurfactant derived from Bacillus subtilis SPB1 versus the phyto -pathogenic fungus, Fusarium solani. Environmental Science and Pollution Research 22 (22): 18137–47. https://doi .org /10.1007/s11356-015-5005-6
O’Brien, Philip A. 2017. Biological Control of Plant Diseases. Australasian Plant Pathology 46 (4): 293–304. https ://doi.org /1 0.1007/s13313-017-0481-4
Ottonelli Stopiglia, Cheila Denise, Fabrício Mezzomo Collares, Fabrício Aulo Ogliari, Evandro Piva, Carmen Beatriz Borges Fortes, Susana Maria Werner Samuel, and Maria Lúcia Scroferneker. 2012. Antimicrobial activity of [2-(methacryloyloxy) ethyl] trimethyl ammonium chloride against candida spp. Revista Iberoamericana de Micologia.https://doi.org/10.1016/j.riam.2011.03.003.
Sha, Yuexia, Qingchao Zeng, and Shuting Sui. 2020. Screening and application of Bacillus strains isolated from non-rhizospheric rice soil for the biocontrol of rice blast. Plant Pathology Journal 36 (3): 231–43. https:// doi. Org /10.5423/PPJ. OA.02. 2020.0028
Shimodat, Mitsuya, and Takayuki Shibamoto. 1990. Isolation and identi fication of headspace volatiles from brewed coffee with an on-column GC/ MS method. Journal of Agricultural and Food Chemistry. https://doi.org /10.102 1/jf00093a045
Smith, Ann C., and Marise A. Hussey. 2005. Gram stain protocols. American Society for Microbiology 1:14
Tian, Yueru, Bing Zheng, Bei Wang, Yong Lin, and Min Li. 2016. Rapid identifycation and multiple susceptibility testing of pathogens from positive-culture sterile body fluids by a combined MALDI-TOF mass spectrometry and vitek susce ptibility system. Frontiers in Microbiology 7 (APR). https://doi.org/ 10.3389/fmicb.00 523
Walters ,D. R., R. L. Walker and K. C. Walker, 2003. Lauric acid exhibits antifungal activity against plant pathogenic fungi. Journal of Phytopatho logy – wiley Online library.
Yang, E. J., and H. C. Chang. 2010. Purification of a new antifungal Compound produced by Lactobacillus plantarum AF1 isolated from kimchi. International Journal of Food Microbiology. https://doi.org/10.1016/j. ijfoodmicro.2010.02.012
Yogeswari, S., S. Ramalakshmi, R. Neelavathy, and J. Muthumary. 2012.Identification and comparative studies of different volatile fFractions from monochaetia kansensis by GC-MS. Global Journal of Pharmacology 6 (2): 65–71
Yuan, Jun, Waseem Raza, Qirong Shen, and Qiwei Huang. 2012. Antifungal activity of Bacillus amyloliquefaciens NJN-6 volatile compounds against Fusarium oxysporum f. sp. cubense. Applied and Environmental Microbiology 78 (16): 5942–44. ttps://doi.org/10.11 28/AEM.01357-12.
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