EFFECIENCY OF COPPER NANOPARTICLES COATED MINT AS ANTI-FUNGAL AGAINST SAPROLEGNIASIS DISEASE IN COMMON CARP

Authors

DOI:

https://doi.org/10.36103/ijas.v53i5.1626

Keywords:

aquaculture, cyprinus carpio, copper sulphate, fungi

Abstract

The present study was aimed to find out the efficacy of M-CuNPs, mint, and CuSO4 in controlling saprolegniasis in common carp. The efficacy of Cu-NP which prepared by using chemical method was examined. 120 fish of weight 80±10g were groups divided into six replications (10 fish/replicate) and the groups were treated as follows: (C-) health without any infection and without treatment, (C+) infected and without treated, (T1 and T2) The fish were infected with the fungus and treated with M-CuNPs at concentrations 20, and 10 mg/l respectivly.T3: fish infected with fungus and treated with copper sulfate at concentration 0.5mg/l. T4: Infected fish were treated at a concentration 100 mg/L of mint. After 14 days of treatment, samples were collected from fish for study the histopathological changes. The copper nanoparticles treatment, 20 mg/l showed the highest survival rate (85%). The survival rate of the control group (C+)(50%). Histopathological studies revealed a significantly increased (p<0.05) percentage of gill epithelial proliferation and epithelial lifting in fish from nanoparticles groups relative to C(+) and C(-). Copper nanoparticles appear to be a good disinfectant against Saprolegnia infection at the dose of 20 mg/l.

References

Alejandro, M., G. Patricio, G. Sebastián, Z. Luis, M. Alejandra, W. Enrique, C. Mauricio, V. Joan, and M. Iván. 2015. Chemical characterization and anti-Oomycete activity of Laureliopsis philippianna essential oils against Saprolegnia parasitica and S. australis. Molecules, 3-8047. 20(5):803

Al-Mahmood, S.S. 2017. Gross and histopathological study on common carp Cyprinus carpio L. diseases in rearing culturing ponds in Kirkuk Province – Iraq. Iraqi Journal of Veterinary Medicine, 41(1):109–117

Al-Rekabi, S.A., R.A. Naeem, and A.N. Butty. 1996. Specifity of baits in isolation Saprolegenia. Al-Mustansiriyah J. Sc., 7(1):20-32

Al-Rudainy, A. J. and H. A. Khalel. 2019. Histopathological changes (gills and liver) and clinical signs of common carp, Cyprinus carpio L. exposed to graphene nanoparticles. Iraqi Journal of Agricultural Sciences, 50(3), 901–908

Amass, S.F., S.L. Glockling, and A. Luis 2001. Evaluation of the efficacy of a peroxygen compound, Virkon™ S, as a boot-bath disinfectant. Journal Swine Health Production, 9(3):121-123

Amlacker, A. 1970. Textbook of Fish Diseases Edited by T. F. H. Publ., Neatune city, New Jersy. pp: 117- 135

Ashour, A.A., N. M. Salman, S.A. Mustafa, and R. O. Nemah. 2019. Evaluation of hydrogen peroxide on controlling saprolegniasis in common carp, Cyprinus Carpio L. Biochemical and Cellular Archives, 19(2): 4247–4252

Bernet, D., H. Schmidt, W. Meier, P. BurkhardtHolm, and T. Wahli.1999. Histopathology in fish: proposal for a protocol to assess aquatic pollution. Journal of Fish Diseases 22: 25-34

Bruno, D.W. and B.P. Wood. 1999. Saprolegnia and Other Oomycetes. In: P.T.K. Woo, D.W. Bruno (Eds.). Fish Diseases and Disorders. Viral, Bacterial and Fungal Iinfections, Vol. 3. CABI Publishing, Wallingford, Oxon, United Kingdom, pp: 599- 659

Bruno, D.W., P. Van West, and G.W. Beakes. 2011. Saprolegnia and other Oomycetes. In P.T.K. Woo and Bruno, D.W. (Eds.), Fish diseases and disorders. Volume 3(18): 669–720

Coker, W.C. 1923. The Saprolegniaceae: With Notes on Other Water Molds.University of North Carolina Press, 201 p

Dixon, J., A. Gulliver, and D. Gibbon. 2001. Farming Systems and Poverty: Improving Farmers Livelihoods in a Changing World. FAO, Rome & World Bank, Washington. D. C Pp: 41

F.A.O. 2014. The State of World Fisheries and Aquaculture. Food and Agriculture Organization of the United Nations, Rome: pp: 233

Hatai, K. and G.I. Hoshiai.1999. Pathogenicity of Saprolegnia parasitica Coker. In: Mueller GJ (ed) Salmon Saprolegniasis. U.S. Department of Energy, Bonneville Power Administration, Portland. Oregon, pp: 87–98

Hernández-Hernández, F., F. García-Gil, A. RojasMartínez, S.Y. Hernández-Martínez, and H. Mendoza-Lanz. 2003. Carminic acid dye from the homopteran Dactylopiuscoccus hemolymph is consumed during treatment with different microbial elicitors. Archives of Insect Biochemistry and Physiology. 54(1): 37-45

Meyer, F.P. 1991. Aquaculture disease and health management. J. Anim Sci. 69(10):4201- 4208

Mohammad, M.A., and W.A. Qasab Bashi. 2020. Effect of partial substitution spirulina instead of soybean meal in common carp Cyprinus carpio L. Diet on some blood picture and some biochemical criteria. Iraqi Journal of Agricultural Sciences, 51(6): 1740–1746

Muhsin, T.M. 1977. Studies of Saprolegniaseae of Shatt Al- Arab. M.Sc. thesis, College of Sciences, University of Basrrah, Iraq, pp: 50

Murray, C.B. , C.R. Kagan, and M.G. Bawendi. 2000. Synthesis and characterisation of monodisperse nanocrystals and close-packed nanocrystal assembliesAnnual Review of Materials Science, 30:545-610

Mustafa, S.A. 2012. An integrated approach to assess impact of environmental stress in carp, Cyprinus carpio L.: Biochemical, genotoxic, histopathological and individual level effects, Ph.D. thesis, University of Plymouth. pp:125

Myers, M.S., L.L. Johnson, T. Hom, T.K. Collier, J.E. Stein, and U.Varanasi, 1998. Toxicopathic hepatic lesions in subadult English sole (Pleuronectes vetuls) from Puget Sound, Washington, USA: Relationships with other biomarkers of contaminant exposure. Marine Environmental Research, 45(21):47-67

Okumuş, İ. 2002. Rainbow trout brood-stock management and seed production in Turkey: present practices, constraints and the future. Turkish J. of Fisheries and Aquatic Sciences, 2(1):41-56

Robert, M.D., J. David, and S.T. Jeffery. 2003. Saprolegniasis (Winter Fungus) and Branchiomycosis of Commercially Cultured Channel Catfish. Southern Regional Aquaculture Center publications no. pp: 4700, 402

Seymour, R.L. 1970. The genus Saprolegnia. Nova Hedwigia, 19(1-2):1-124

Soltani, M.E., M. Esfandiary, M. Sajadi, S. Khazraeenia, A.R. Bahonar, and H. Ahari. 2010. Effect of nano-particles on hatchability of rainbow trout (Oncorhynchus mykiss) egg and survival of the produced larvae. Iranian Journal of Fisheries Sciences, 10(1):167-176

Song, M.F. , Y.S. Li , H. Kasai, and K. Kawai. 2012. Metal nanoparticle-induced micronuclei and oxidative DNA damage. Journal of Clinical Biochemistry and Nutrition, 50:211-216

Zaki, M.S., O.M. Fawzi, and J. El-Jackey. 2008. Pathological and biochemical studies in Tilapia nilotica infected with saprolegnia parasitica and treated with potassium permanganate. American-Eurasian Journal of Agriculture and Environmental Sciences: 3(5):677-680.

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2022-10-25

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How to Cite

Alwash, S. W., Al-Faragi, J. K., & Al-Saadi, T. M. (2022). EFFECIENCY OF COPPER NANOPARTICLES COATED MINT AS ANTI-FUNGAL AGAINST SAPROLEGNIASIS DISEASE IN COMMON CARP . IRAQI JOURNAL OF AGRICULTURAL SCIENCES, 53(5), 1129-1137. https://doi.org/10.36103/ijas.v53i5.1626

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