EXTRACTION, PURIFICATION AND CHARACTERIZATION OF LIPASE FROM THE DIGESTIVE DUCT OF COMMON CARP CYPRINUS CARPIO L.

Authors

  • Hind. K. Ali
  • J. M. Nasser
  • K. A. Shaker

DOI:

https://doi.org/10.36103/ijas.v53i5.1615

Keywords:

lipase activity, Ion exchange, gel filtration, molecular weight.

Abstract

This study was aimed to use digestive duct of common carp Cyprinus Carpio L. as economic source for fish  lipase extraction . The enzyme was extracted using potassium phosphate buffer (pH 7 , 0.2 M ). The crude extract then precipitated at the saturation range between 40 – 80 % and dialyzed . Further purified carried out  through ion exchange chromatography using DEAE-Cellulose column  (2.5 × 30 cm) and gel filtration chromatography by Sephadex G-100 column  (1.5 x 60 cm). The purified lipase had  a molecular weight of 37.150KDa ,and optimum pH and temperature for enzyme activity about 45◦C and 6.5 using olive oil as substrate .The specific activity, number of purification folds and yield of purified Lipase were 146.48U/mg, 9.76 and 40.50% respectively.             

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Published

2022-10-25

How to Cite

Ali, H. K., Nasser, J. M. and Shaker, K. A. (2022) “ PURIFICATION AND CHARACTERIZATION OF LIPASE FROM THE DIGESTIVE DUCT OF COMMON CARP CYPRINUS CARPIO L”., IRAQI JOURNAL OF AGRICULTURAL SCIENCES, 53(5), pp. 1011–1020. doi: 10.36103/ijas.v53i5.1615.

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