CASPASE 9 GENE EXPRESSION AND CASPASE 9 PROTEIN IN CHRONIC MYELOID LEUKEMIA IN IRAQ

Authors

  • Abdullah W. K.
  • Maha F. A.

DOI:

https://doi.org/10.36103/ijas.v53i5.1613

Keywords:

patients, age, gender, healthy, chronic myeloid

Abstract

The following study was carried out to investigate gene expressions of the CASPASE 9 gene and CASPASE 9 protein with the development of chronic myeloid leukemia (CML) in Iraq. The differences in the expression of this gene between patients and healthy controls were included. The correlation of age and gender with CML occurrence comparing with controls was studied. Results showed significant increases in the mean of gene expression level (ΔCt) of patient groups compared to the corresponding ΔCt means in control group, also the gene expression folding (2-∆∆Ct) of  CASPASE 9  gene  reflect significant differences in the expression showed the highest level in CML patients which reached to (16.19), the level of CASPASE 9 protein was (1.025 ng/ml) in patients with CML compared to control groups with significant differences.  On the other hand no significant differences were exhibited according to age and gender between CML patients and control, this result suggest that CML can affect all ages and occur in both male and females.

References

Abdulhasan, G. A. 2021.Lemon grass essential oil shows an antiproliferative effect on lung cancer cells and its role in expression of p53 gene. Iraqi Journal of Agriculture Sciences.52 (2):359-366

Albano, F., A. Zagaria, L. Anelli, N. Coccaro, L. Impera, CF. Minervini, A. Minervini, AR. Rossi, G. Tota, P. Casieri and G. Specchia, 2013. Gene expression profiling of chronic myeloid leukemia with variant t (9; 22) reveals a different signature from cases with classic translocation.” Molecular Cancer; 4 May. 12- 36

Altaee, M. F., R. W. Younis and Z. K. Kamona 2020. Activity of Annona squamosa peels extract against two pathogenic bacteria and two blood cancer cell lines. Iraqi Journal of Agriculture Sciences .51 (6): 1496-1503

Arbise, J. L., M.Y. Bonner and L.C. Gilbert 2017. Targeting the duality of cancer.NPJ Precision Oncology, 1(1), 1–7

Buchdunger, E., A. Matter and B.J. Druker 2001. Bcr-Abl inhibition as a modality of CML therapeutics.Biochim Biophys Acta. ; 1551(1):11-8

Chang, H.Y. and X. Yang 2000. Proteases for cell suicide: functions and regulation of caspases, Microbiol. Mol. Biol. Rev. 64 (4):821–846

Duaa, A. S. 2020.Genetic Polymorphisms of Caspase 8 and 9 Genes in Myeloid Leukemia of Iraqi Patients, M.Sc. thesis. University of Baghdad. ‏College of Science.64-65

Elsahookie, M.M., S. H. Cheyed and A. A. Dawood 2021. An overview on mechanism of gene expression regulation. Iraqi Journal of Agriculture Sciences. 52(2):454-460.

Ercan, S., S. Arinc, S. yilmaz, C. Altunok, F. Yaman and T. Isbir 2019. Investigation of caspase 9 gene polymorphism in patients with non-small cell lung cancer.Anticancer Research; 39 (5) 2437-2441

Extermann, E., and U. Wedding 2012. Comorbidity and geriatric assessment for older patients with hematological malignancies. A Review of the Evidence. J Ger Oncol; 3, 49-57

Forthofer, R. N., and E.S. Lee 2014. Introduction to biostatistics: a guide to design, analysis, and discovery. Elsevier.pp 323-331

Gunnarsson, N., M. Hoglund, L. Stenke, S. Wållberg-Jonsson, F. Sandin, M. Björkholm, A. Dreimane, M. Lambe, B. Markevärn, U. Olsson-Strömberg, H. Wadenvik, J. Richter, and A. Själander 2016. Increased prevalence of prior malignancies and autoimmune diseases in patients diagnosed with chronic myeloid leukemia. Leukemia; 30(7):1562-7

Hofny, E.R.M., H.I. Hasan, N.A. Mohamed, M.S. Bamatraf and A.M.M. Mahran 2017. Bcl-2, active caspase-9 and inactive caspase-9 levels as markers of apoptosis in the serum and tissue of vitiligo patients. Al-Azhar Assiut Medical Journal. 15 (2): 85-91

Hossain, M. S., M. S. Iqbal, M. A. Khan, M.G. Rabbani, H. Khatun, S. Munira, MM. Miah, AL. Kabir, N. Islam, TF. Dipta, F. Rahman, A. Mottalib, S. Afrose, T. Ara, AR. Biswas, M. Rahman, AM. Abedin, M. Rahman, AB. Yunus, LW. Niessen and TA. Sultana 2014.Diagnosed hematological malignancies in Bangladesh - a retrospective analysis of over 5000 cases from 10 specialized hospitals. BMC Cancer; 14; 14:438

Iłżecka. J. 2012. Serum caspase-9 levels are increased in patients with amyotrophic lateral sclerosis. Neurol Sci.; 33(4): 825–829

Jbireal, JM., EA. Azab, S. Alzahani and M. Elshareef 2019. Haematological and cytogenetic changes in CML patients treated with imatinib mesylate in Western Libya.Hematology and Transfusion International Journal 7(3): 50‒57

Johnstone, RW, AA. Ruefli and SW. Lowe 2002. Apoptosis: a link between cancer genetics and chemotherapy. Cell; 108(2):153–64.

Kalkavan, H., and D.R. Green 2018. MOMP, cell suicide as a BCL-2 family business. Cell Death and Differentiation, 25(1), 46–55

Kenneth, J. and M. Thomas 2001. Analysis of Relative Gene Expression Data Using RealTime Quantitative PCR and the 2-DDCT Method. Methods; 25: 402–408

Kim, B., Sanjay, KS, and K. Sung-Hoon 2014. Caspase-9 as a therapeutic target for treating cancer. Expert Opinion on Therapeutic Targets; 19(1): 113-127

Koenig, A., Russell, J.Q., Rodgers, W. A., and R.C. Budd 2008. Spatial differences in active caspase-8 defines its role in T-cell activation versus cell death. Cell Death and Differentiation, 15(11), 1701–1711

Melo, JV, Hughes, TP., and JF. Apperley 2003. Chronic Myeloid Leukemia. ASH Education Program Book; (1):132–152

Pasquini,MC. 2012. Hematopoietic cell transplantation for chronic myeloid leukemia in developing countries: perspectives fro

Latin America in the post-tyrosine kinase inhibitor era. Hematology; 17(Suppl 1):S79-82

Patel, N. and N. Prakash 2013. Principle and Tools for Primer Design. Atmiya. Spandan. 1: 79 -95

Qin, A., J. Zhu, X. Liu, D. Zeng, M. Gu and C. Lv 2017. MicroRNA‐1271 inhibits cellular proliferation of hepatocellular carcinoma. Oncol Lett; 14(6): 6783–6788

Reis, C., H. Reis, C. Araujo, C. Russell, S. Welsh, A. Sharip and P. Kim 2017. Inhalational chemical exposure resulting in myelogenous leukemia. International Journal of Biomedical Research; 8 (02): 108-110

Shaw, J., and LA. Kirshenbaum 2006. HAX-1 represses post-mitochondrial caspase-9 activation and cell death during hypoxia-reoxygenation.Circ Res.99:336–338

Sordet, O., C. Rébé, S. Plenchette, Y. Zermati, O. Hermine, W. Vainchenker, C. Garrido, E. Solary and L. Dubrez-Daloz 2002. Specific involvement of caspases in the differentiation of monocytes into macrophages. Blood; 100: 4446–4453

Wurstle, M.L, M.A. Laussmann and M. Rehm 2012. The central role of initiator caspase-9 inapoptosis signal transduction and theregulation of its activation and activityon the apoptosome. Exp Cell Res; 318(11):1213-208.

Downloads

Published

2022-10-25

How to Cite

K., A. W. and A., M. F. (2022) “ CASPASE 9 GENE EXPRESSION AND CASPASE 9 PROTEIN IN CHRONIC MYELOID LEUKEMIA IN IRAQ”, IRAQI JOURNAL OF AGRICULTURAL SCIENCES, 53(5), pp. 994–1002. doi: 10.36103/ijas.v53i5.1613.

Issue

Section

Articles