LINK BETWEEN SOME VIRULNCE FACTORS GENES AND ANTIBACTERIAL RESISTANCE OF PSEUDOMONAS AERUGINOSA
DOI:
https://doi.org/10.36103/ijas.v53i5.1612Keywords:
Pseudomonas aeruginosa, virulence factors genes, antibacterial resistance.Abstract
The research was aimed to demonstrate the frequency of virulence factors genes (exoenzyme S and exotoxin A) and to express their relationship to antibacterial resistance among Pseudomonas aeruginosa isolated from patients suffering from otitis externa. The results were revealed that only twenty one (32%) out of 65 clinical ear swabs were Pseudomonas aeruginosa. According to PCR amplification, 18 (85.7%) bacterial isolates were expressed both toxins ( exoenzyme S and exotoxin A). The data get by real time experiments were revealed that the isolates were give higher percentage of resistance were seen against Ceftazidime (90.5%) and Gentamicin (88.5%).Only thirteen isolates (61,90%) of Multidrug resistance (MDR) –Pseudomonas aeruginosa isolates were detected, and two (9.5%) isolates were resist to all 8 classes of antibiotics and considered as PDR, and three (14.2%) bacterial isolates resist seven classes of antibiotics which considered as XDR.
References
Berthelot P, I. Attree, P. Plesiat and et al. 2003: Genotypic and phenotypic analysis of type III secretion system in a cohort of Pseudomonas aeruginosa bacteremia isolates: evidence for a possible association between O serotypes and exo genes. J Infect Dis188:512–8. [PubMed] [Google Scholar].
Bilavsky E, I. Pfeffer, I. J. Tarabeia and et al. 2013: Outbreak of multidrug- resistant Pseudomonas aeruginosa infection following urodynamic studies traced to contaminated transducer. J Hosp Infect; 83:344.
Breidenstein EBM, C. de la Fuente-Núñe, and R. Hancock 2011: Pseudomonas aeruginosa: all roads lead to resistance. Trends Microbiol J. 19:419– 426..04.005. [PubMed] [CrossRef] [Google Scholar]
Coburn J, AV. Kane, L. Feig, and DM. Gill 1991: Pseudomonas aeruginosa exoenzyme S requires a eukaryotic protein for ADP-ribosyl transferase activity. J Biol Chem 266: 6438–6446.
Cosgrove, S. E and Y. Carmeli. 2003: The impact of antimicrobial resistance on health and economic outcomes. Clin. Infect. Dis. 36:1433-1437. [PubMed] [Google Scholar]
David R. H, M. R. T. Helena. Parracho, James Walker, S.G.Richard., W, Maria., L, Susan. and M, Sandra. 2014: Bacteriophages and Biofilms Antibiotics. 3, 270-284
ECDC. 2015. Antimicrobial resistance surveillance in Europe. 2015: Annual report of the European Antimicrobial Resistance Surveillance Network (EARS-Net). European Centre for Disease Prevention and Control
Forbes, A, F. Sahm and S. Weissfeld, Bailey and Scott’s. 2007: Diagnostic Microbiology. 12th Edition, Mosby Elsevier, China, 842-855
Ijaz T, AA. S. Anjum, S. Aslam, SA. Raja, AR. Khawaja and S. Ljaz. 2014 : Microbial profiling and risk factors assessment for Otitis Media and Otitis Externa. Advancements in Life Sciences. 191-196
Tong Wu, I and Zhu, Jianwei . 2021: Recent development and optimization of pseudomonas aeruginosa exotoxin immunotoxins in cancer therapeutic applications. Int. Immunopharmacol; l ; 96:107759
Martin TJ, JE. Kerschner and VA. Flanary. 2005: Fungal causes of otitis externa and tympanostomy tube otorrhea. Int J Pediatr Otorhinolaryngol.; 69(11):pp. 1503–8. [PubMed] [Google Scholar].
Musso MF and JD. Crews. 2016: Infections of the External Ear. Infectious Diseases in Pediatric Otolaryngology.: pp. 15-28
Naqi SA. 2016: Microbiology of Cerumen Bacterial Flora of Acute Otitis Externa Patients.20: pp. 144-146
National Committee for Clinical Laboratory Standard (NCCLS). 2020. Performance standards for antimicrobial susceptibility testing: twelfth informational supplement. NCCLS document M100-S12.PA, USA
Neher A, M. Nagl and AW. Scholtz. 2008: Otitis externa. HNO ;56:1067– 1080. [PubMed] [Google Scholar].
Oliver A, X. Mulet, C. López-Causapé and C, Juan. 2015. The increasing threat of Pseudomonas aeruginosa high-risk clones. Drug Resist Update 21–22:41– 59. [PubMed] [CrossRef] [Google Scholar].
Pederson, K. J, S. Pal, A. J. Vallis, D. W. Frank, and J. T. Barbieri. 2000. Intracellular localization and processing of Pseudomonas aeruginosa ExoS in eukaryotic cells. Mol. Microbiol. 37:287-299.
Pena C, G. Cabot, S. Gomez-Zorrilla and et al. 2015: Influence of virulence genotype and resistance profile in the mortality of Pseudomonas aeruginosa bloodstream infections. Spanish Network for Research in Infectious Diseases (REIPI). Clin Infect Dis 60:539–548
Poole K. 2011: Pseudomonas aeruginosa: resistance to the max. Front Microbiol 2:65. Published online 2011 Apr 5. Prepublished online 2011 Mar 18. doi: 10.3389/fmicb.2011.00065 [PMC free article] [PubMed] [CrossRef] [Google Scholar]
Roland PS and DW. Stroman. 2002: Microbiology of acute otitis externa. Laryngoscope. 112 (7):1166–77. [PubMed] [Google Scholar].
Rowshan HH, K. Keith, D. Baur and P. Skidmore P 2008 : Pseudomonas aeruginosa infection of the auricular cartilage caused by “high ear piercing”: A case report and review of the literature. J Oral Maxillofac Surg.; 66 (3):543– 6. [PubMed] [Google Scholar].
Sader HS, M. Castanheira , LR . Duncan and RK.Flamm. 2018. Antimicrobial susceptibility of Enterobacteriaceae and Pseudomonas aeruginosa isolates from United States medical centers stratified by infection type: results from the International Network for Optimal Resistance Monitoring (INFORM) surveillance program. Diag. Microbial Infect. Dis 92:69–74
Schaefer P and RF. Baugh. 2012:.Acute otitis externa: an update. Am Fam Physician; 86:1055.
Tartor YH and EY. El-Naenaeey . 2016 : RT-PCR detection of exotoxin genes expression in multidrug resistant Pseudomonas aeruginosa. Cell Mol Biol (Noisy-le-grand). Jan 22;62(1):56-62
Vincent, T. S, J. E. Fraylick, E. M. McGuffie, and J. C. Olson. 1999. ADP- ribosylation of oncogenic Ras proteins by Pseudomonas aeruginosa exoenzyme S in vivo. Mol. Microbiol. 32:1043-1053. [PubMed] [Google Scholar].
Walkty A, P. Lagace-Wiens and H. Adam . 2017: Antimicrobial susceptibility of 2906 Pseudomonas aeruginosa clinical isolates obtained from patients in Canadian hospitals over a period of 8 years: Results of the Canadian Ward surveillance study (CANWARD), 2008–2015. Diagn Microbiol Infect Dis 87:60–63
Wiegand, S, R. Berner, A. Schneider, E. Lundershausen and A. Dietz, 2019 : Otitis Externa: Investigation and Evidence-Based Treatment. Dtsch Arztebl Int. 116(13): 224–234
L. Q. Jawad and H. A. R. R. Rasheed. 2002: isolation and purification of anticancer protein exotoxin from Pseudomonas aeruginosa . Iraqi Journal of Agricultural Sciences Vol. 53 No. 1
Tawfiq S. M.2018. Bacteriologigal and genetic study of Pseudomonas aeruginosa isolates. Iraqi Journal of Agricultural Sciences, 49:1
Younis, R. W. 2020: Production, purification and inhibition of alginate lyase from local isolate of pseudomonas aeruginosa na11. Iraqi Journal of Agricultural Sciences. Vol. 51 No. 6.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
How to Cite
2.jpg)
2.jpg)
