IMPACT OF AQUEOUS EXTRACT OF NEEM LEAVES IN LOWERING BLOOD GLUCOSE AND LIPID PROFILE IN STZ INDUCED DIABETES MELLITUS MICE
DOI:
https://doi.org/10.36103/ijas.v53i5.1611Keywords:
hypoglycemia, Azadirachta indica, cholesterol, body weight.Abstract
This study was aimed to investigate the effect of aqueous neem (Azadirachta indica) leaves extract in reducing the sugar level of diabetic patients in male mice. The study included four groups and 15 mice for each group. The first group represented the control group. The mice in the remaining three groups were injected with streptozotocin (STZ) at dose of 50 mg/kg body weight. The second group was considered as the induced control group for diabetic patients for comparison, while the third group of diabetic patients received neem leaves extract 250 mg/kg of body weight, and the fourth group of diabetic patients was given metformin hydrochloride 250 mg/kg of body weight. Measured Changes in blood glucose, blood triglycerides (TG), total cholesterol (TC), HDL, LDL cholesterol, VLDL cholesterol levels, and body weight. The results showed there was a significant decrease in blood glucose levels in diabetic groups and a decrease in cholesterol and triglycerides in groups treated with neem leaves extract compared with metformin hydrochloride. Thus, it can be concluded high blood sugar can be alleviated by treatment with an aqueous extract of Azadirachta indica leaves.
References
Alanbari, E. H. and H. J. Mohamed. 2017. Determination of insulin gene insg polymorphisms and their relationship with some productive traits in both sexes of hybrid Broiler Ross308. The Iraqi Journal of Agricultural Sciences; 0830-0833: (6) 48.
AL-Attaby , A. K. and M. Q. D. AL-lami. 2019. Role of Calcuim-regukation Hormones, adipocytokines and renal function test in the progress of type 2 diabetes mellitus in a sample of Iraqi Patients. Iraqi Journal of Agricultural Sciences ;50(1):343-352
Al-Hamdani, H., M. S. 2019. Effect of stevia leaves consumption on sugar and other blood characters in diabetes-induced mice. Iraqi Journal of Agricultural Sciences. 50(6):1652-1660
Al-Hamdani, H., M. S. Al- S.A. Timmemi, Muhammed and J.Raheem. 2019. physiological and histopathological study for the effect of barly(Hordeun Vulgare)flour on induced diabetic. Iraqi Journal of Agricultural Sciences.50 (6):1635-1644
Andallu,A.Vinay Kumar and N.Ch. Varadacharyulu.2009.Lipid abnormalities in streptozotocin-diabetes: Amelioration by Morus indica L. cv Suguna leaves. Int J Diabetes Dev Ctries ; 29(3): 123–128
Appleby RN, I.Moghul, S.Khan,and et al.2019. Non-Alcoholic fatty liver disease is associated with dysregulated bile acid synthesis and diarrhea: a prospective observational study. PLoS One; 25;14(1).
Bailey CJ.2017. Metformin: historical overview. Diabetologia ; 60:1566–76
Barua CC, A.Talukdar, AG. Barua, A. Chakraborty, RK. Sarm, and RS. Bora. 2010. Evaluation of the wound healing activity of methanolic extract of Azadirachta Indica (Neem) and Tinosporacordifolia (Guduchi) in rats. PharmacologyOnline; 1:70–77
Chattopadhyay RR, Bandyopadhyay M.2005. Effect of Azadirachta indica leaf extract on serum lipid profile changes in normal and streptozotocin induced diabetic rats. Afr J Biomed Res; 8:101–104
Duangjai A, N. Nuengchamnong, LH. Lee, BH. Goh, S. Saokaew and N. Suphrom.2017. Characterisation of an extract and fractions of Azadirachta indica flower on cholesterol lowering property and intestinal motility. Nat Prod Res; 33(1):1-4
Ezeigwe OC, CJ. Ononamadu, BN. Enemchukwu, UF. Umeoguaju and JC. Okoro. 2015. Antidiabetic and Antidiabetogenic properties of the aqueous extracts of neem leaves on alloxaninduced diabetic wistar rats. International Journal of Biosciences; 7(2):116–126
Ference BA, JJP. Kastelein, KK.Ray KK, and et al. 2019. Association of Triglyceride-lowering lPL variants and lDL-C-lowering lDLR variants with risk of coronary heart disease. JAMA; 321:364–373
Gale EA. 2013.Lessons from the triglitazones. A story of drug development. Lancet ;357:1870–5
Ganjali S, GM.Dallinga-Thie, Simental-LE. Mendia, M.Banach, M. Pirro and A. Sahebkar. 2017. HDL functionality in type 1 diabetes. Atherosclerosis; 267:99–109
Gheibi, S., K. Kashfi and A. Ghasemi. 2017. A practical guide for induction of type-2 diabetes in rat: Incorporating a high-fat diet and streptozotocin. Biomedicine and Pharmacotherapy; 95, 605–613
Hiroshi Takemori, Akie Hamamoto, Kenta Isogawa,et al. 2020.Mouse model of metformin-induced. BMJ Open Diab Res Care; 8(1).
Jenna I. Wurster,L. Rachel and E. Peterson Brown ,and et al. 2021. Streptozotocin-induced hyperglycemia alters the cecal metabolome and exacerbates antibiotic-induced dysbiosis. Cell Reports; 37, 110113
Khaleel Nagarchi, Saif Ahmed, Anusha Sabus and Shaik Hussain Saheb.2015. Effect of Streptozotocin on Glucose levels in Albino Wister Rats. /J. Pharm. Sci. and Res; 7(2), 67-69
Lee JW, HW. Ryu, SY.Park, et al.2017. Protective effects of neem (Azadirachta indica A. Juss.) leaf extract against cigarette smokeand lipopolysaccharide-induced pulmonary inflammation. Int J Mol Med; 40(6):1932-1940
Margit Solyma , Ivan Ivic , La´szlo´ Po´to , Pe´ter Hegyi, and et al.2018. Metformin induces significant reduction of body weight, total cholesterol and LDL levels in the elderly – A meta-analysis. PLoS ONE; 13(11).
Mariël F. van, Albert A. Graaf and Albert K. Groen. 2018. Actions of metformin and statins on lipid and glucose metabolism and possible beneft of combination therapy. Cardiovasc Diabetol; 15(1):81
Morris J, CB. Gonzales, JJ. De La Chapa, and et al.2019. The highly pure neem leaf extract, SCNE, inhibits tumorigenesis in oral squamous cell carcinoma via disruption of pro-tumor inflammatory cytokines and cell signaling. Front Oncol. VOL: 9(890).
Muhammad Zafar and Syed Naeem-ul-Hassan Naqvi. 2010. Effects of STZ-Induced diabetes on the relative weights of kidney, liver and pancreas in albino rats: A Comparative Study. Int. J. Morphol., 28(1):135-142
Obiajulu C. Ezeigwe, Francis C. Ezeonu, Chukwudi O. Okani, Daniel and N. Onwusulu. 2020. Aqueous extract of azadirachta indica leaves favorably alters the course of streptozotocin-induced diabetes in rats: A comparative prospective cohort study, Biomedical Research and Therapy; 7(7):3877-3889
Olga Horakova, Petra Kroupova, Kristina Bardova, and et al. 2019. Metformin acutely lowers blood glucose levels by inhibition of intestinal glucose transport. Department of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences; 9:6156.
Patel MJ, S. Tripathy, KD. Mukhopadhyay,and et al. 2018. A supercritical CO2 extract of neem leaf (A. indica) and its bioactive liminoid, nimbolide, suppresses colon cancer in preclinical models by modulating pro-inflammatory pathways. Mol Carcinog; 57(9):1156–1165
Saidu Y, SA. Muhammad, AY.Abbas, A.Onu , IM. Tsado and L. Muhammad. 2017. In vitro screening for protein tyrosine phosphatase 1B and dipeptidyl peptidase IV inhibitors from selected nigerian medicinal plants. Journal;6(1):1
SAS. 2012. Statistical Analysis System, User's Guide. Statistical. Version 9.1th ed. SAS. Inst. Inc. Cary. N.C. USA
Sathyanarayan S,and KS. Pillai. 2019. Antidiabetic and antidyslipidemic properties of goa-111, a mixture of gymnemasylvestrae, ocimum sanctum and azadirachta indica extract in the ratio of 1:1:1 studied in high fat diet fed- low dose streptozotocin induced experimental type 2 diabetes in rat. J Drug Deliv Ther; 9(4-A).
Shahla Rezaei , Fatemeh Ashkar , Farhad Koohpeyma , and et al .2020. Hydroalcoholic extract of Achillea millefolium improved blood glucose, liver enzymes and lipid profile compared to metformin in streptozotocin-induced diabetic rats. Lipids in Health and Disease; 19:81
Shayaa, W A. H. and A. Hussein.2019. Effect of neem leaves extract and organig fertilizer in the productivity and quality of two potato cultivars. Iraqi Journal of Agricultural Sciences; 50(1):275- 285
Sheikhpour R. 2013.Diabetes and oxidative stress: the mechanism and action. Iranian J Diabetes Obesity; 5(1):40 –5
Shradha B and S. Sisodia. 2010.www. ijrap. netInt J Res Ayurveda Pharm;1(1):33 42
Shrivastava A, U. Chaturvedi, R.Sonkar, AK.Khanna, JK.Saxena and G. Bhatia. 2012.Antioxidant effect of Azadirachta indica
on high fat diet induced diabetic charles foster rats. Appl Biochem Biotechnol; 167(2):229-36
Sitasiwi AJ, S. Isdadiyanto and SM. Mardiati. 2018.Effect of ethanolic Neem (Azadirachta indica) leaf extract as an herb contraceptive on Hepato-somatic Index of the male mice (Mus musculus) J Phys: Conf Series; 1(2): 108-113
Suryawanshi N, A. Bhutey, A.Nagdeote, A.Jadhav,and G.Manoorkar. 2006. Study of lipid peroxide and lipid profile in diabetes mellitus. Indian J Clin Biochem; 21(1):126
Teresa M. Braga, Lídia Rocha, Tsz Yan Chung, and et al.2021. Azadirachta indica A. Juss. In Vivo Toxicity—an Updated Review. Molecules; 26, 252
Usharani Pingali, Mohammed Abid, Ali Srinivas Gundagani, and et al .2020. Evaluation of the effect of an aqueous extract of azadirachta indica (Neem) leaves and twigs on glycemic control, endothelial dysfunction and systemic Inflammation in Subjects with Type 2 diabetes mellitus – a randomized, double-blind, placebo-controlled clinical study. diabetes, metabolic syndrome and obesity: Targets and Therapy; 13: 4401–4412
Yibcharoenporn C, P.C. Chusuth, Jakakul, and et al.2019. Discovery of a novel chalcone derivative inhibiting CFTR chloride channel via AMPK activation and its anti-diarrheal application. J Pharmacol Sci; 140:273–83
Zafar, M.; Naeem-ul-Hassan S. Naqvi, M.Ahmed and Z.A. Kaim Khani. 2009.Altered liver morphology and enzymes in streptozotocin-induced diabetic rats. Int. J. Morphol; 27(3):719-25.
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