ASSESSMENT OF THE SUCCESS OF MICRO GRAFTING CLEMENTINE TIMING ON SOUR ORANGE
Keywords:Grafting, In vitro, Citrus, Marine algae extract, Salicylic acid.
This study was carried out at three locations (Laboratory of Plant Tissue Culture, Fields of the College of Agriculture, University of Diyala and one of the private nursery at the city of Baqubah. The applied experiments included two separate experiments on the scion Mandarin Clementine micro grafted on Sour orange rootstock. The first micro grafting experiment aimed to develop a method of vegetative propagation of Clementine and transferring the micro grafts from a laboratory to the field and comparing grafting dates (spring, early autumn, late autumn) with the laboratory grafting after treating grafting region with different concentrations of gibberellin (0, 0.3 or 0.4 mg. L-1). The effect of grafting dates on the percentages of success of grafted plants after one month of acclimatization. The experiment was carried out according to completely randomized design (CRD) as a factorial experiment with two factors and three replications for the first one, and as a single factor experiment with three replicates for the second experiment. Results showed a decline in the percentage of success of the grafting and a slight success correlated with increased gibberellin concentration in the aforementioned experiment at early fall grafting. A second field experiment, was conducted with the aim of studying the success of maintained micro propagated plants during summer season and the effect of foliar spray with salicylic acid (0, 200 or 400 mg. L-1) and marine algae extract (0, 5 or 10 g. L-1) on some characteristics of vegetative growth and some chemical characteristics. The experiment was carried out according to the Randomized Complete Block Design (RCBD), as a factorial experiment with two factors and three replications. No significant effect for both factors was recorded in most vegetative characteristics, while chemical composition was significantly affected caused by the two factors.
- Al-Hawezy, S. M. N. 2014. The use of Kelpak to seedlings loquat Eriobotya Jappanica L. Inter. J. Sci. Res. Publi. 4(5):1-4
- Al-hayany, A. M. Abd; AL-Sarah, E. M.; Hathal, N. M. 2017. Effect of foliar application salicylic acid on citrus rootstocks tolerance to soil salinity. The Iraqi Journal of Agricultural Sciences 48(3): 212-207
- Al-Sahaf, F. H.; Y. M.; Abed, F. F. Saleh, and H. S. M. Khierallah, 2017. Influence of spraying by some plant growth regulators on date palm Salt tolerance enhancement. The Iraqi Journal of Agricultural Sciences 48(1): 236-241
- Castle, W. S., R. R. Pelosi, C. O. Youtsey, F. G. Gmitter, J. R., R. F. Lee, C. A. Powell, and X. Hu. 1992. Rootstocks similar to sour orange for florida citrus trees. Proc. Fla. State Hort. Soc. 105: 56-60
- Darikova, Julia A.; V. Yulia Savva; A. Eugene Vaganov; M. Alexi Grachev and V. Galina Kuznetsova. 2011. Grafts of woody plants and the problem of incompatibility between Scion and Rootstock (a review). J. Siber. Fed. Univ. Biol. 4(1):54-63.
- David, O., S. Collin, J. Sievert and M. Lu Arpaia, 2013. Mandarin flavor and aroma volatile composition are strongly influenced by holding temperature. Postharvest Biol. and Tech. 82: 6–14
- Gainza, Felipe; Opazo, Ismael and Muñoz, Carlos. 2015. Graft incompatibility in plants: Metabolic changes during formation and establishment of the rootstock/scion union with emphasis on Prunus species. Chilean J. Agric. Res. 75(1): 28-34
- Hartmann, H. T.; D. E. Kester; F. T. Davies; and R. L. Geneve. 2014. Plant Propagation Principles and Practices. Printice - Hall, Inc. Upper Saddle River, New Jersey. USA
- Hedden, P. and Y. Kamiya. 1997. Gibberellin biosynthesis: Enzymes. Genes and their regulation. Annu. Rev. Plant. physiol. plant 1401. Biol. 48: 431-460
- Hoopkins, W. G. and N. P. Muner. 2008. Introduction to Plant Physiology. 4th ed. Wiley and Sons. USA
- Juarez, J.; M. I. Molins; L. Navarro. and N. Duran-Vila. 1990. Separation of citrus viroid by shoot-tip grafting in vitro. Plant Path. 39: 472-476
- Kapari-Isaia, T.; G. J. Minas; D. Polykarpou; E. Iosephidou; S. Arseni and A. Kyriakou. 2002. Shoot-tip grafting in vitro for elimination of viroids and citrus psorosis virus in the local “ Arakapas” mandarin in Cyprus. Fifteenth IOCV Conference. 417-419
- Keita, H. and Y. Noguchi. 1991. Productive conditions of virus-free plant by heat treatment and shoot-tip grafting together on Satsuma mandarin. Bull. Fukuoka Agric. Res. Cent. 11: 81-84
- Martin, J. 2012. Impact of marine extracts applications on CV. syrah grape (Vitis vinifera L.) yield components, harvest juice quality parameters, and nutrient uptake. A Thesis, The faculty of California Polytechnic State University, San Luis Obispo
- Morales-Payan, J. P. and J. Norrie. 2010. Accelerating the growth of Avocado (Persea americana) in the nursery using a soil applied commercial extract of the brown alga Ascophyllum nodosum. International Seaweed Symposium. pp. 189
- Murashige, T. and D. P. H. Tucker. 1969. Growth factor requirement of citrus tissue culture. Proc. 1st Int. Citrus Symp. 3: 1155-1161
- Murashige, T.; Bitters, W. R.; Rangan, E. M.; Nauer, E. M. and C. N. Roistacher. 1972. A technique of shoot apex grafting and its utilization towards recovering virus-free citrus clones. Hort. Sci. 7: 118-119
- Navarro, L. 1984. Citrus tissue culture. In: Micropropagation of selected root Crop, palms, citrus and ornamental species. Plant production and protection. 59. FAO. U.N. Rome. 113-154
- Navarro, L.; C. N. Roistacher. and T. Murashige. 1975. Improvement of shoot-tip grafting in vitro for virus-free citrus. J. Amer. Soc. Hort. Sci. 100(5): 471-479.
- Pasquale, F. D.; S. Giuffrida and F. Carimi. 1999. Mini grafting of shoots, roots, inverted roots and somatic embryos for rescue of in vitro citrus regenerants. J. Amer. Soc. Hort. Sci. 124(2): 152-157.
- Smith, R. H. 2013. Plant Tissue Culture: Techniques and Experiments. Academic Press
- Taha, F. H. and M. R. Abood, 2018. Influence of some organic fertilizers on date palm cv. Barhi 1. Vegetative growth. Agricultural Sciences 49(4):632- 638.
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