microproth, phenol content, theymol, essential oils, bactericidal effect


The antibacterial activity of essential oils extracted from Origanum compactum, Thymus capitatus, Foeniculum vulgare, and Rosmarinus officinalis was assessed with the well diffusion method and a microbroth dilution assay against E. coli isolated from the carcasses of rabbits. The chemical composition of these essential oils was also determined by gas chromatography coupled with mass spectrometry (GC/MS). The results of this study indicate that essential oils with high phenol content exert a strong antibacterial activity against E. coli. Essential oils of Origanum compactum and Thymus capitatus containing high amounts of the monoterpenoid phenols thymol and carvacrol (68.99% and 95.25% carvacrol composition, respectively) were particularly effective against E. coli  with low values of MIC = 0.3125% v/v and MBC = 0.625% v/v to report. The essential oil of Foeniculum vulgare also possessed  moderate antibacterial activity (MIC = 50 % v/v) with a non-bactericidal effect, while the essential oil of Rosmarinus officinalis was ineffective at the concentrations tested.


-Ait-Ouazzou A., L., Espina L., Cherrat M., M., Hassani A., Laglaoui P.Conchello and R., Pagán 2012. Synergistic combination of essential oils from Morocco and physical treatments for microbial inactivation. Innovative Food Science and Emerging Technologies. 16, 283-290

-Badgujar S. B., V. V.Patel and A. H. Bandivdekar ., 2014. Foeniculum vulgare Mill: A Review of its botany, phytochemistry, pharmacology, contemporary application, and toxicology. BioMed Res Int 2014. http://dx.doi.org/10.1155/2014/842674

-Bakhy K. O, A. Benlhabib J. Bighelli , F. Casanova and C. Tomi . 2014. Yield and chemical variability of the essential oil isolated from aerial parts of wild Origanum compactum Benth. from Moroccan Western Rif. Am J Essent Oils Nat Prod; 1(4): 1-9

-Bouhdid, S., S. N. Skali, M. Idaomar, A. Zhiri, D. Baudoux, M. Amensour, and J. AbriniJ., 2008. Antibacterial and antioxidant activities of Origanum compactum essential oil. Afr. J. Biotechnol. 7:1563–1570.

-Bouhdid S, J. Abrinin, A. Zhiri , M. J Espuny and A. Munresa . 2009. Investigation of functional and morphological changes in Pseudomonas aeruginosa and Staphylococcus aureus cells induced by Origanum compactum essential oil. Journal of Applied Microbiology.; 106: 1558-1568

-Bounatirou S, S. Smiti, MG Miguel, M.N.Rejeb M.Neffati M, M.M.Costa , L. Faleiro AC. Figueiredo JG., Barroso JG and L.G. Pedro . 2007. Chemical composition, antioxidant and antibacterial activities of the essential oils isolated from Tunisian Thymus capitatus Hoff. Et Link. Food Chemistry. 105: 146-155

-Carlet J., V. Jarlier , S. Stephan Harbarth , A. Voss , H. Goossens and D. Pittet . 2012. Ready for a world without antibiotics? The Pensières Antibiotic Resistance Call to Action Antimicrobial Resistance and Infection Control. 1:11. http://www.aricjournal.com/content/1/1/11

-Chedia A., H. Ghazghazi , S. Dallali , S. Houssine , H. Brahim and M. Abderazzak 2013 Comparison of Chemical Composition, Antioxidant and Antimicrobial Activities of Thymus capitatus L. essential Oils from two Tunisian localities (Sousse and Bizerte). International Journal of Agronomy and Plant Production. 4, 1772-1781

-De M., A. K. De ., R. Mukhopdhyay M., Miro and A. B. Anerjee . 2001. Actividad antimicrobiana de Illicium verum Hook. f. Antimicrobial actions of Illicium verum Hook. Ars Pharmaceutica, 42:3-4; 209-220

-Djeddi, S., N. Bouchenah, I. Settar, . and H.D. Skaltsa . 2007. Composition and antimicrobial activity of the essential oil of Rosmarinus officinalis from Algeria. Chemistry of Natural Compounds 43(4): 487-490

-Džamić A.M , B.J.Nikolić , A.A. Giweli , D.S.Mitić-Ćulafić , M.D.Soković , MS. Ristić , JB. Knežević-Vukčević and PD.Marin . 2015. Lybian Thymus capitatus essential oil: antioxidant, antimicrobial, cytotoxic and colon pathogen adhesion-inhibition properties. Journal of Applied Microbiology ; 119(2):389-99

-Ennabili A, N. Gharnit and EL. El Hamdouni . 2000. Inventory and social interest of medicinal, aromatic and Honey-plants from Mokrisset region (NW of Morocco). Ediciones Universidad de Salamanca 19: 57-74.

-El Ouariachi E.M., J. Paolini , A. Bouyanzer , P. Tomi ., B. Hammouti , R. Salghi , L. Majidi and J. Costa ., 2011. Chemical Composition and Antioxidant Activity of Essential Oils and Solvent Extracts of Thymus capitatus (L.) Hoffmanns and link from Morocco. Journal of Medicinal Plants Research, 5: 5773-5778

-Gulfraz M., S. Mehmood, N. Minhas , N. Jabeen , R. Kausar , K. Jabeen , and G. Arshad . 2008. Composition and antimicrobial properties of essential oil of Foeniculum vulgare. African Journal of Biotechnology 7 : 4364-4368

-Goren AC, G. Bilsela, M. Bilsela , H. Demira and E.E. Kocabas ., 2003. Analysis of essential oil of Coridothymus capitatus (L.) and its antibacterial and antifungal activity. Z. Naturfo. B., 58: 687-690

-Grigore A., S. Colceru-Mihul, S. Paraschivi, Nita , R. Christof , R. Iuksel and M. Ichim ., 2012. Chemical analysis and antimicrobial activity of indigenous medicinal species volatile oils. Romanian Biotechnological Letters 17, No.5

-Hammer K. A., C. F. Carson . and T.Y. Riley . 1999. Antimicrobial activity of essential oils and other plant extracts. Journal Of Applied Microbiologiy, 86, 985-990

-Issabeagloo E., P. Kermanizadeh , M. Taghizadieh and K. Forughi , 2012. Antimicrobial effects of rosemary (Rosmarinus officinalis L.) essential oils against Staphylococcus spp. African Journal of Microbiology Research . 6(23), 5039-5042

-Javed S., S. Mushtaq , L. Khokhar , R. Ahmad and M.S. Haider . 2012. Comparative antimicrobial activity of clove and fennel essential oils against food borne pathogenic fungi and food spoilage bacteria. African Journal of Biotechnology 11 : 16065-16070

-Lambert R.J.W., P.N. Skandamis , P.J. Coote and G.J.E.Nychas ., 2001. A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. Journal of Applied Microbiology, 91, 453-462

-Mann C and J. Markham . 1998 A new method for determining theminimum inhibitory concentration of essential oils. J Appl Micro-biol 84:538–44

-Mathlouthi N., T. I. Bouzaienne, F. Oueslati, M. Recoquillay, M. Hamdi, M. Urdaci and R. Bergaoui., 2012. In vitro antimicrobial activities and effects on growth performance Use of rosemary, oregano, and a commercial blend of essential oils in broiler chicken. Journal of Animal Science. 90:813-823

-Megdiche-ksouri Saada M., B. Soumaya , M. Snoussi , Y. Zaoulali and R. Ksouri , 2015. Potential use of wild Thymus algeriensis and Thymus capitatus as source of antioxidant and antimicrobial agents Journal of New Sciences, Agriculture and Bi otechnology, 23(4), 1046-1056

-Milon A., E. Oswald and J. De Rycke,1999. Rabbit EPEC: a model for the study of enteropathogenic Escherichia Coli . Vet res 30, 203-219

-Mounchid K., F. Bourjilat , N. Dersi , T. ,Aboussaouira , A. Rachidai ,A. Tantaoui-, A. Elaraki and M. Alaoui-Ismaili . 2005. The susceptibility of Escherichia coli strains to essential oils of Rormarinus officinalis and Eucalyptus globules. African Journal of Biotechnology Vol. 4 (10), pp. 1175-1176. http://www.academicjournals.org/AJB

-Moujahed N., Y. Bouaziz , A. Ben Jannet and Z. Ghrabi . 2011. Nutritive value and essential oils characterization of Rosmarinus officinalis and Thymus capitatus from the central region of Tunisia. Options Méditterranéennes, 99, 245-249

-Mourey, A.; and N.Canillac, . 2002. Anti-Listeria monocytogenes activity of essential oils components of conifers. Food Control, 13 : 289-29.

-Prabuseenivasan S., M. Jayakumar and S. Ignacimuthu ., 2006. In vitro antibacterial activity of some plant essential oils. BMC Complementary and Alternative Medicine, 6:39. http://www.biomedcentral.com/1472-6882/6/39

-Prescott L. M, J. P. Harley and D. A. Klein . 1995. Microbiologie. De Boeck ed. pp: 1014

-Remmal A., T.Bouchikhi , K. Rhayour and M. Ettayebi . 1993. Improved methode for the determination of antimicrobial activity of essential oils in agar medium. J. Essent . Oil Res. 5: 179-184

-Shahat A.A., A.Y. Ibrahim , S.F. Hendawy , E.A. Omer . F.M., Hammouda , F.H. Abdel-Rahman and M.A. Saleh .2011. Chemical composition, antimicrobial and antioxidant activities of essential oils from organically cultivated fennel cultivars. Molecules, 16: 1366-1377.

-Sienkiewicz M., M. Łysakowska, M. Pastuszka , W. Bienias and E. Kowalczyk . 2013. The Potential of Use Basil and Rosemary Essential Oils as Effective Antibacterial Agent. Molecules. 18, 9334 -9351

-Stefanini, M. B., L. C. Ming, M. O. M. Marques , R. Facanali , M.A.A. Meireles, , L.S.Moura, J.A., Marchese, and L.A. Sousa, . 2006. Essential oil constituents of different organs of fennel (Foeniculum vulgare var. vulgare). Rev. Bras. Pl. Med. 8, 193–198

-Tani Z.B., M. A. Khelil , M. Bendahou and M. Mestari . 2009. Action des huiles essentielles de trois plantes aromatiques de l’Ouest algerien sur la mite Tineola Bisselliella. Rev. Ivoir. Sci. Technol., 14: 175 – 186

-Vimal M., P.P. Vijaya , P. Mumtaj and M.S. Seema Farhath . 2013. Antibacterial activity of selected compounds of essential oils from indigenous Plants. J. Chem. Pharm. Res., 5(1):248-253

-Wassenaar T.M., 2005. – The use of antimicrobial agents in veterinary medicine and implications for human health. Crit. Rev.Microbiol., 31, 155–169.

-Xu J. F., Zhou , B.P. Ji , R.S. Pei and N. Xu . 2008. The antibacterial mechanism of carvacrol and thymol against Escherichia coli. Letters in Applied Microbiology 47, 174–179.




How to Cite

B, M. ., Barchan, A., Aarab, A., Bakkali, M. ., Arakrak, A. . and Laglaoui, A. . (2022) “EVALUATION OF THE ANTIBACTERIAL ACTIVITY OF ESSENTIAL OILS AGAINST E. COLI ISOLATED FROM RABBITS”, IRAQI JOURNAL OF AGRICULTURAL SCIENCES, 53(4), pp. 802–809. doi: 10.36103/ijas.v53i4.1592.