INVESTIGATE GENETIC RELATION AMONG WATERMELON CULTIVARS USING MOLECULAR DNA MARKERS
DOI:
https://doi.org/10.36103/ijas.v53i3.1581Keywords:
crimson sweet, sugar baby, charleston gray, genetic diversity.Abstract
This study was aimed to investigate genetic relation among 21 selective of watermelon seeds which collected from different commercial companies for different origins and produced at different years by using 12 primers for Inter Simple Sequence Repeat (ISSR). The results showed that the selective of Crimson sweet had genotypes highest genetic relation between ( K1 and K2) it was found less than the genetic similarity between ( K1and K4) , ( K2 and K4) .The four selective were divided two groups A and B. The results showed that the selective a Charleston gray had the highest genetic relation between (CH4 and CH6) while it found less than the genetic relation between (CH5 and CH9). The ten selectives were divided two cluster A and B,The groups A was divided three sub clusters .The results revealed that the highest genetic relation between the selective of the variety Sugar baby (S1and S3). While found less genetic relation between the (S1 and S5) and divided the selectives of Sugar baby into two main cluster A and B, The cluster B was divided two sub cluster. The result found that the highest genetic relation among the 21 selective it was between ( CH4 and CH6), while found less than of genetic similarity among ( CH8 and S5) ( CH8 and S2),( S2 and K1) ,( S2 and K2) ,( S2 and CH9),( K1 and S5) ( S5 and CH9). The 21 selectives was divided two main clusters A and B the group A was divided to three sub clusters while the group B was divided two sub clusters. It could be concluded that the genetic relation it is very important in the hybridization programs to it prove a large genetic base to benefit of plant breeding programs.
References
Abdel-Ghani, A. H. and A. Mahadeen, 2014. Genetic Variation in Snake melon (Cucumis melo var. flexuosus) populations from Jordan using morphological traits and RAPDs . Jordan Journal of Agricultural Sciences, 10 (1): 96-119.
Alsohim, A.S. and M.I. Motawei. 2014. Genetic diversity and presence of DREB gene in watermelon cultivars and wild typeof watermelon based on molecular markers. Journal of Food, Agriculture and Environment .12 (3&4): 2 8 1 - 2 8 4.,3. Ashter, S. 2009.Evaluation of some Genotyps of the Syrian Wheat (hexagonal and quaternary) using Biochemical and Molecular Markers are Different. Ph.D. Dissertation Agronomy Department. Faculty of Agriculture. University of Tthishreen. Syria. pp: 192.,4. Al-Burki, F. R.. 2020 . Plant Breeding and Improvement. Ministry of Higher Education and Scientific Research. faculty of Agriculture . Al-Muthanna University. Iraq.pp:401.,5. Ammiraju, J.S.S., B. B. Dholakia, D. K. Santra,., H. Singh, M. D. Lagu and M.D., Tamhankar and et al. 2001. Identification of inter simple sequece repeat (ISSR) markers associated with seed size in wheat. Theor. Appl. Genet. 102:726-732.,6. Arifiyanti, R., 2015. Variasi Genetik Tanaman Melon (Cucumis melo L.) Berdasarkan Penanda Molekuler Inter-Simple Sequence Repeat. Undergraduate Thesis, Faculty of Biology, Universitas Gadjah Mada.,7. Blair, M. W., O. Panaud and S. R. McCouch, .1999. Inter-simple sequence repeat (ISSR) amplification for analysis of microsatellite motif frequency and fingerprinting in rice (Oryza sativa L.). Theor. Appl. Genet. 98: 780-792.,8. Bornet, B., and M. Branchard,. 2001. Nonanchored intersimple sequence repeat (ISSR) markers: reproducible and specifics tools for genome fingerprinting. Plant Mol.Biol. Rep. 19: 209–215.,9. Bornet, B., F. Goraguer, G. Joly and M. Branchard,. 2002. Genetic diversity in European and Argentinian cultivated potatoes (Solanum tuberosum subsp.tuberosum) detected by inter-simple sequence repeats (ISSRs). Genome 45:481-484.,10. Cavan, G., V. Potier and S. R. Moss. 2000. Genetic diversity of weeds growing in continuous wheat. Weed Res. 40: 301-310.,
Che,K., C.Liang,Y.Wang, D.Jin and B.wang,.2003.Genetic assessment of watermelon germplasm using the AFLP Technique. Hort Science 38(1):81-84.
Eleuch, L., A. Jilal, S.Grando, S.Ceccarelli, M.V.K.Schmising, H.Tsujimoto, A.Hajer, A. Daaloul, and M.Baum,. 2008. Genetic diversity and association analysis for salinity tolerance, heading date and plant height of barley germplasm using simple sequence repeat markers. Journal integrative Plant Biology. 8: 1004 - 1014.
Elias, M. S. 2016. Distinguish among some selective watermelons by using ISSR technology. The Iraqi Journal of Agricultural Sciences – 47(5):1235-1245.
Fang, D. Q. and M. L. Roose,.1997. Identification of closely related citrus cultivars with inter-simple sequence repeat markers. Theor. Appl. Genet. 95, 408-417.
Jain, A., C. Apparanda and P. L. Bhalla. 1999. Evaluation of genetic diversity and genome fingerprinting of Pandorea (Bignoniaceae) by RAPD and inter-SSR PCR. Genome 42: 714-719.
Jena, R.C. and P.K. Chand. 2021. Multiple DNA marker-assisted diversity analysis of Indian mango (Mangifera indica L.) populations. Scientific Reports. 11, 10345.
Karbin, M., A. Kuras and E. Żurawicz. 2002. Fruit plant germplasm characterisation using molecular markers generated in RAPD and ISSRPCR.Cell. Mol. Biol. Lett., 785-794.
Levi, A. and C. E. Thomas, T. C. Wehner, and X. Zhang .2001. Low genetic diversity indicates the need to broaden the genetic base of cultivated watermelon. hortscience 36(6):1096–1101.
Ministry of Planning. Central Statistical Organization. 2020. Production of Secondary Crops and Vegetables by Governorates. Agricultural Statistics Directorate.
Mualla, M. Y. and Harba, N.A. 1994. Genetic improvement of fruit and vegetable trees. Publications of the collage of Agriculture, Tishreen University. Latakia. Syrian Arab Republic.pp: 278.
Nagaraju, J., M. Kathirvel, R. Ramesh Kumar, E. A. Siddiq and S. E. Hasnain,.2002. Genetic analysis of traditional and evolved Basmati and non-Basmati rice varieties by using fluorescence-based ISSR-PCR and SSR markers.Proc. Natl. Acad. Sci. U.S.A. 99: 5836-5841.
Nei, M. and W.H. Lei, 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA., 76: 5269-5273.
Ng, W.L. and S.G.Tan. 2015. Inter simple sequence repeat (ISSR) marker.ASM Science Journal. 9(1):30-39.
Pandey,A. M. K. Khan, R. Isik, O. Turkmen, R. Acar, M. Seymen, and E. E. Hakki. 2019. Genetic diversity and population structure of watermelon (Citrullus sp.) genotypes.3 Biotech. 9(6): 210
Qian. Z., D. Hong and Z. Dong Hang,. 2007. ISSR Molecular marker and its application in plant researches. Molecular Plant Breeding, .5(6):123-129
Raina, S. N., V. Rani, T. Kojima, Y. Ogihara, K. P. Singh and R. M. Devarumath,. 2001. RAPD and ISSR fingerprints as useful genetic markers for analysis of genetic diversity, varietal identification, and phylogenetic relationships in peanut (Arachis hypogaea) cultivars and wild species.Genome 44, 763- 772
Rakoczy-Trojanowska, M. and H. Bolibok,. 2004. Characteristics and a comparision of three classes of Micro satellite- based markers and their application in plants. Agricultural, Nowoursynowska,-Wraszawa, Poland.
Robinson, R. W. and D. S. Decker- Walters,. 1997. Cucurbits. CAB international, Wallingford, U. K.pp : 240
Salsabila, A.J., A.S.Subiastuti and B.S.Daryono.2021.Identification of ISSR-based molecular markers mssociated with ploidy level of orange watermelon(Citrullus lanatus) (Thunb.) matsum and nakai.Tropical Biodiversity and Biotechnology.6(3):1-8
Sambrook J, E.F. Fritsch, and T. Maniatis,. 1989. Molecular Cloning: a Laboratory Manual. NY: Cold Spring Harbor.pp:545
Statsoft, Inc.1999. SPSS (Statistical Package of Social Science). Data Analysis Software System .Version 10.
Soghani,Z.N., M. Rahimi, M. A. Nasab and M. Maleki,2018. Grouping and genetic diversity of different watermelon ecotypes based on agro-morphological traits and ISSR marker .Iheringia Série Botânia. 73(1): 53–59.
Stone,S. and G. Boyhan.2019. Inter- and intracultivar variation of heirloom and open-pollinated watermelon cultivars.hortscience.54(2):212–220.
Varshney,R.K.,D.A.Hoisington,S.N.Nayak and A.Graner. 2009.Molecular plant breeding: methodology and achievements. Plant Genomics. 513: 283-290
Wang, J.Y., Y.M. Chang, B.K.Shen, Y.H.Wang, P.C.Liou, . 2005. Molecular markers derived from RAPD and ISSR analysis for identification of watermelon. J. Taiwan Agric. Res. 54, 257–269
Williams, J.G.K., A.R Kubelik., K.J.Livak, J.A.Rafalski and S.V.Tingey 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acids Res.18: 6531-6535
Wolfe, A.D., Q.Y. Xiang and S. R. Kephart,. 1998. Assessing hybridization in natural populations of penstemon (Scrophulariaceae) using hypervariable inter simple sequence repeat markers. Mol. Ecol. 71:1107-1125
Wu,S., X. Wang, U. Reddy, H. Sun, K. Bao, L. Gao, L. Mao, T. Patel, C. Ortiz, V. L. Abburi, P. Nimmakayala, S. Branham, P. Wechter, L. Massey, K. Ling, C. Kousik, S. A. Hammar, Y. Tadmor, V. Portnoy, A. Gur, N.Katzir, N. Guner, A. Davis, A. G. Hernandez, C.. L. Wright, C. McGregor, R. Jarret, X. Zhang, Y. Xu, T. C. Wehner, R. Grumet, A.Levi and Z. Fei. 2019. Genome of ‘Charleston Gray’, the Principal Americanwatermelon Cultivar, and Genetic Characterization of 1,365 accessions in the U.S. National Plant Germplasm Systemwatermelon Collection. Plant Biotechnology. pp. 1–13
Zietkiewicz, E., A. Rafalski and D. Labuda,. 1994. Genome fingerprinting by simple sequence repeat (SSR) –anchored polymerase chain reaction amplification. Genomics 20: 176-183.
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