BIOLOGICAL CONTROL OF THE COMPLEX DISEASE OF Rhizoctonia solani AND ROOT-KNOT NEMATODE Meloidogyne javanica ON CHICKPEA BY Glomus spp. AND Pseudomonas sp.
DOI:
https://doi.org/10.36103/ijas.v53i3.1577Keywords:
PPO; POD enzymes; rhizobacteria; rhizoctonia-meloidogyne complex disease; arbuscular mycorrhizal fungi; cicer arietinumAbstract
Rhizoctonia-Meloidogyne complex disease is a serious problem facing legume production in many countries. The infection of chickpea (Cicer arietinum) by pathogens Rhizoctonia solani (R) and Meloidogyne javanica (M) in a single infection or combination causes severe damage to plant growth. The effect of using Glomus spp. (G) and Pseudomonas sp. (P) as a biological control agent against Rhizoctonia- Meloidogyne complex disease was tested and assessed by comparing the growth and disease parameters in infected and control plants. Chickpea growth parameters are characterized by measuring shoot and root length, and fresh and dry weight. The results of using (G) and (P) in a single treatment or in combination showed a decrease in the root gall index and in root rot disease severity when compared to the infected and healthy control treatments. The (M+ R+ G) and (M + R + G + P) combination treatment increased polyphenol oxidase (POD) and peroxidase (PPO) enzyme activity value as well as the total phenols content in treated chickpea roots. The combined effects of (G) and (P) on the pathogens’ progression and the positive effects on chickpea growth parameters are suggested to be involved in efficient disease control.
References
Abood, I. D. and F. A. Fattah 2012. Effect of Planting Date and Disease Complex on The Productivity of Iraqi Wonder and Barcelona Eggplant Cultivars Under Field Conditions. Iraqi Journal of Agricultural Sciences. 43(2) (Special Issue): 27-33.
Al-Hazmi, A. S., and S. N. Al-Nadary, 2015. Interaction between Meloidogyne incognita and Rhizoctonia solani on green beans. Saudi Journal of Biological Sciences, 22(5): 570-574.
Aljawasim, B. D. G., H. M. Khaeim and M. A. Manshood 2020. Assessment of arbuscular mycorrhizal fungi (Glomus spp.) as potential biocontrol agents against damping-off disease Rhizoctonia solani on cucumber. Journal of Crop Protection, 9(1): 141-147.
Al-Waily, D.S., L. A. Al-Saad and S. S. Al-Dery 2018. Formulation of Pseudomonas Fluorescens as a Biopesticide Against Soil Borne Root Pathogens. Iraqi Journal of Agricultural Sciences, 49(2): 235-242.
Aoyagi, T., K. Kageyama and M. Hyakumachi 1998. Characterization and survival of Rhizoctonia solani AG2-2 LP associated with large patch disease of zoysia grass. Plant Disease, 82(8): 857-863.
Bajestani, M. S., E. M. Moghadam, R. Aghnoum, and H. Rohani 2019. Genotypic and Biochemical variation in the response of barley to the Root-knot nematode (Meloidogyne javanica) at seedling stage. Pakistan Journal of Phytopathology, 31(1): 07-17.
Baker, K. R. 1985. Nematode extractions and bioassays. In: An advanced treatise on Meloidogyne. Vol. II. Methodology, eds. by Baker, K.R., C.C. Carter, and J.N. Sasser,. North Carolina State University, NC, USA.
Castillo, P., J. A. Navas-Cortés, B. B. Landa, R. M. Jiménez-Díaz, and N. Vovlas, 2008. Plant-parasitic nematodes attacking chickpea and their in-planta interactions with rhizobia and phytopathogenic fungi. Plant Disease, 92(6): 840-853.
El‐Tarabily, K. A. 2004. Suppression of Rhizoctonia solani diseases of sugar beet by antagonistic and plant growth‐promoting yeasts. Journal of Applied Microbiology, 96(1): 69-75.
Ganeshamoorthi, P., and S. C. Dubey 2015. Morphological and pathogenic variability of R. solani isolates associated with wet root rot of chickpea in India. Legume Research-An International Journal, 38(3): 389-395.
Hussey R.S. and K. R. Barker 1973. A comparison of methods of collecting inocula of Meloidogyne spp. including a new technique. Pl. Dis. Rept., 57: 1025-1028.
Jacott, C. N., J. D. Murray, and C. J. Ridout 2017. Trade-offs in arbuscular mycorrhizal symbiosis: disease resistance, growth responses and perspectives for crop breeding. Agronomy, 7(4): 75.
Karajeh, M.R. 2015. Checklist of host range of root-knot Nematodes (Meloidogyne species and races) in Jordan. Jordan Journal of Agricultural Sciences., 11(3): 761-769.
Khan, M. R., F. A. Mohidin, U. Khan, and F. Ahamad 2016. Native Pseudomonas sp. suppressed the root-knot nematode in in vitro and in vivo, and promoted the nodulation and grain yield in the field grown Mungbean. Biological Control, 101: 159-168.
Matloob, A. A. H., A. Y. Abid and K. Z. Khadhair 2017. Efficiency of arbuscular mycorrhizal fungi and some species of plant growth promoting Rhizobacteria to control fungi Fusarium chlamydosporum causing agent of decline date palm off shoots. Iraqi Journal of Agricultural Sciences, 48(2): 507- 519.
Pitotti, A., B. E. Elizalde, and M. Anese 1994. Effect of caramelization and Maillard reaction products on peroxidase activity. Journal of Food Biochemistry, 18(6): 445-457.
Roorkiwal, M., A. Rathore, R. R. Das, M. K. Singh, A. Jain, S. Srinivasan and R. K. Varshney 2016. Genome-enabled prediction models for yield related traits in chickpea. Frontiers In Plant Science, 7: 1666.
Singh, H. B., C. Keswani, M. S. Reddy, E. Sansinenea, and C. García-Estrada (Eds.). 2019. Secondary metabolites of plant growth promoting rhizomicroorganisms: discovery and applications. Springer Nature Singapore Pte Ltd. Pp.404.
Sohrabi, F., M. Sheikholeslami, R. Heydari, S. Rezaee and R. Sharifi 2020. Investigating the effect of Glomus mosseae, Bacillus subtilis and Trichoderma harzianum on plant growth and controlling Meloidogyne javanica in tomato. Indian Phytopathology, 73(2): 293-300.
War, A. R., M. G. Paulraj, M. Y. War and S. Ignacimuthu 2011. Jasmonic acid-mediated-induced resistance in groundnut (Arachis hypogaea L.) against Helicoverpa armigera (Hubner)(Lepidoptera: Noctuidae). Journal of Plant Growth Regulation, 30(4): 512-523.
Wood, C. W., B. L. Pilkington, P. Vaidya, C. Biel and J. R. Stinchcombe 2018. Genetic conflict with a parasitic nematode disrupts the legume–rhizobia mutualism. Evolution letters, 2(3): 233-245.
Yanan, W., Z. Xusheng, Y. Baozhong, Z. Wenchao, and G. Jintang 2015. Biochemical defenses induced by mycorrhizae fungi Glomus mosseae in controlling strawberry fusarium wilt. The open biomedical engineering journal, 9: 301.
Zhao, D., H. Zhao, D. Zhao, X. Zhu, Y. Wang, Y. Duan and L. Chen 2018. Isolation and identification of bacteria from rhizosphere soil and their effect on plant growth promotion and root-knot nematode disease. Biological control, 119: 12-19.
Zieslin, N., and R. Ben Zaken 1993. Peroxidase activity and presence of phenolic substances in peduncles of rose flowers. Plant Physiology and Biochemistry (Montrouge), 31(3): 333-339.
Zwart, R. S., M. Thudi, S. Channale, P. K. Manchikatla, R. K. Varshney and J. P. Thompson 2019. Resistance to plant-parasitic nematodes in chickpea: Current status and future perspectives. Frontiers in Plant Science, 10: 966.
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