ROLE OF RND-EFFLUX PUMP IN LEVOFLOXACIN RESISTANCE AMONG ACINETOBACTER BAUMANNII
DOI:
https://doi.org/10.36103/ijas.v53i3.1562Keywords:
disc diffusion method, gene expression, real-time PCR, MIC.Abstract
In this study out of 130 different clinical specimens, 70 Acinetobacter baumannii were collected from hospitalized patients. Then; all the isolates were characterized using biochemical tests and confirmed by using the Vitek II compact system. All isolates of Acinetobacter baumannii were subjected to the disc diffusion method to determine it's a susceptibility to 8 types of antibiotic disc, and the result showed these bacteria were resistant to Cefepime, Imipenem, trimethoprim, Piperacillin, tetracycline, Amikacin, ampicillin-sulbactam and levofloxacin. According to bacterial susceptibility test, all isolated were showed highly resistance to Trimethoprim (88.57%) and the lower resistance to tetracycline (47.14%). Minimum inhibitory concentration was determined only for levofloxacin resistant isolates of Acinetobacter baumannii using the broth dilution method. MIC shows these bacteria were resistant to levofloxacin in concentration ranging between 32µg/100µl to 128µg/100µl. The relation between adeIK and levofloxacin resistance was calculated by determining gene expression using real-time PCR. The result showed the mean value of adeI and adeK before exposing bacteria to levofloxacin was 2.3 and 0.42 respectively while the mean value of adeI and adeK after exposing bacteria to levofloxacin was 3.15 and 3.29 respectively. This study indicates that adeI has a non-significant role (p-value=0.11) in providing resistance to levofloxacin, while adeK plays a significant role (p-value= 0.008) in providing resistance to levofloxacin.
References
Abdi, S. N., R. Ghotaslou, K. Ganbarov, A. Mobed, A. Tanomand, M. Yousefi, M. Asgharzadeh, and H. S. Kafil. 2020. Acinetobacter baumannii Efflux Pumps and antibiotic resistance. Infection and Drug Resistance, 13, 423–434. https://doi.org/10.2147/IDR.S228089
Antunes, L. C., P. Visca, and K. J. Towner. 2014. Acinetobacter baumannii: evolution of a global pathogen. Pathogens and disease, 71(3), 292–301.
https://doi.org/10.1111/2049-632X.12125
Al-haideri, H. H. 2019. Gene expression of blaoxa-51-like and blaoxa-23 in response to β-lactam antibiotic in clinically isolated Acinetobacter baumannii and Acinetobacter lowffii from urine samples. Iraqi Journal of Agricultural Sciences, 50(4). https://doi.org/10.36103/ijas.v50i4.757
Al-Shaabani M. J. M., A. M. T. Al-Ethawi, and H. J.F. AL-Mathkhury. 2020. Eco-frienly synthesis of gold nanoparticles and study their effect with antibiotics against Acinetobacter baumannii. Iraqi Journal of Agricultural Sciences, 51(4), 1204-1211. https://doi.org/10.36103/ijas.v51i4.1099
Basatian-Tashkan, B., M. Niakan, M. Khaledi, H. Afkhami, F. Sameni, S. Bakhti, and R. Mirnejad. 2020. Antibiotic resistance assessment of Acinetobacter baumannii isolates from Tehran hospitals due to the presence of efflux pumps encoding genes (adeA and adeS genes) by molecular method. BMC Research Notes, 13(1), 543. https://doi.org/10.1186/s13104-020-05387-6
Clinical, Laboratory. Standards Institute. 2020. Performance Standards for Antimicrobial Susceptibility Testing. M100–S30.
Coyne, S., N. Rosenfeld, T. Lambert, P. Courvalin, and B. Périchon. 2010. Overexpression of resistance-nodulation-cell division pump AdeFGH confers multidrug resistance in Acinetobacter baumannii. Antimicrobial agents and chemotherapy, 54(10), 4389–4393. https://doi.org/10.1128/AAC.00155-10
Damier-Piolle, L., S. Magnet, S. S. Brémont, T. Lambert, and P.Courvalin. 2008. AdeIJK, a resistance-nodulation-cell division pump effluxing multiple antibiotics in Acinetobacter baumannii. Antimicrobial Agents and Chemotherapy, 52(2), 557–562. https://doi.org/10.1128/AAC.00732-07
De Rosa, F. G., S. Corcione, N. Pagani, and G. Di Perri. 2015. From ESKAPE to ESCAPE, from KPC CCC. Clinical infectious diseases: an official publication of the Infectious Diseases Society of America, 60(8), 1289–1290. https://doi.org/10.1093/cid/ciu1170
Eze, E. C., H. Y. Chenia, and M. E. El Zowalaty. 2018. Acinetobacter baumannii biofilms: effects of physicochemical factors, virulence, antibiotic resistance determinants, gene regulation, and future antimicrobial treatments. Infection and Drug Resistance, 11, 2277–2299. https://doi.org/10.2147/IDR.S169894
Hamza M.R. and L. A. Yaaqoob. 2020. Evaluation the effect of green synthesis titanium dioxide nanoparticles on Acinetobacter baumannii isolates. Iraqi Journal of Agricultural Sciences, 51(6), 1486-1495. https://doi.org/10.36103/ijas.v51i6.1176
Hujer, K. M., A. M. Hujer, E. A. Hulten, S. Bajaksouzian, J. M. Adams, C. J. Donskey, and R. A. Bonomo. 2006. Analysis of antibiotic resistance genes in multidrug-resistant Acinetobacter spp. isolates from military and civilian patients treated at the walter reed army medical center. Antimicrobial Agents and Chemotherapy, 50(12), 4114-4123
Kanafani, Z. A., N. Zahreddine, R. Tayyar, J. Sfeir, G. F. Araj, G. M. Matar, and S. S. Kanj. 2018. Multi-drug resistant Acinetobacter species: a seven-year experience from a tertiary care center in Lebanon. Antimicrobial resistance and infection control, 7, 9. https://doi.org/10.1186/s13756-017-0297-6
Lin, M. F., Y. Y. Lin, C. C. Tu and C. Y. Lan. 2017. Distribution of different efflux pump genes in clinical isolates of multidrug-resistant Acinetobacter baumannii and their correlation with antimicrobial resistance. Journal of Microbiology, Immunology, and Infection = Wei mian yu gan ran za zhi, 50(2), 224–231. https://doi.org/10.1016/j.jmii.2015.04.004
Magnet, S., P. Courvalin, and T. Lambert. 2001. Resistance-nodulation-cell division-type efflux pump involved in aminoglycoside resistance in Acinetobacter baumannii strain BM4454. Antimicrobial Agents and Chemotherapy, 45(12), 3375–3380. https://doi.org/10.1128/AAC.45.12.3375-3380.2001
Monem, S., B. Furmanek-Blaszk, A. Łupkowska, D. Kuczyńska-Wiśnik, K. Stojowska-Swędrzyńska, and E. Laskowska. 2020. Mechanisms protecting Acinetobacter baumannii against multiple stresses triggered by the host immune response, antibiotics, and outside host environment. International Journal of Molecular Sciences, 21(15), 5498
Munier, A. L., L. Biard, M. Legrand, C. Rousseau, M. Lafaurie, J. L. Donay, R. Flicoteaux, A. Mebazaa, M. Mimoun, and J. M. Molina. 2019. Incidence, risk factors and outcome of multi-drug resistant Acinetobacter baumannii nosocomial infections during an outbreak in a burn unit. International journal of infectious diseases: IJID : official publication of the International Society for Infectious Diseases, 79, 179–184. https://doi.org/10.1016/j.ijid.2018.11.371
Wang, T., V. Costa, S. G. Jenkins, B. J. Hartman, and L. F. Westblade. 2019. Acinetobacter radioresistens infection with bacteremia and pneumonia. ID Cases, 15, e00495. https://doi.org/10.1016/j.idcr.2019.e00495
Xu, C., S. R. Bilya, and W. Xu. 2019. adeABC efflux gene in Acinetobacter baumannii. New Microbes and New Infections, 30, 100549. https://doi.org/10.1016/j.nmni.2019.100549
Yoon, E. J., P. Courvalin, and C. Grillot-Courvalin. 2013. RND-type efflux pumps in multidrug-resistant clinical isolates of Acinetobacter baumannii: major role for AdeABC overexpression and AdeRS mutations. Antimicrobial Agents and Chemotherapy, 57(7), 2989–2995. https://doi.org/10.1128/AAC.02556-12.
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