• F. D. Salim
  • K. M. Ibrahim
  • W. H. Yousif



pomegranate, phytochemical analysis, wound healing activity.


The present study was conducted to evaluate wound induced in rabbit skin by using alcohols and aqueous extracts of pomegranate seeds. Total flavonoids concentration were estimated using high performance liquid chromatography (HPLC). The highest flavonoids concentration were recorded in ethanol extract. Acute inflammation was induced in laboratory animals (rabbits) by injecting 0.1mL of 2.5% formalin inside the wound incision. Aqueous and ethanol extracts of pomegranate seeds an anti-inflammatory and healing effect compared with the Vaseline on rabbit skin. A histopathological study was conducted to observe healing development of the induced wound which proved that healing of the skin was obvious by vanishing of odema and decrease in scar size, enhancement of fibroblast proliferation, collagen regeneration, Keratinization and Epithelialization. Extracts  have the ability to reduce the inflammation that induced by formalin and appeared to be efficient in healing wounded rabbits skin.


Abdul, W., G. Mehreen, J. B. Syed, N. Muhammad, K. Ajmal, G. Rukhsana, and F. Asnad. 2013. Phytochemical analysis of medicinal plants occurring in local area of Madam. Biochem. Anal. Biochem., 2 (4): 1-5.

Ali, S. I., F. El-emary, E. A. Khan, and A. Amal. 2014. HPLC-analysis of polyphenolic compounds and free radical scavenging activity of pomegranate fruit (Punica granatum). Int. J. Pharm. Clin. Res., 6 (4): 1-5

AL-Khafaji, .J. O., M. M. F. Al-Halbosiy, Y. A. Hadi, S. M. AL-Chalabi, and B. A. Abdulhameed 2018. Antimicrobial effect of crude frog (Rana ridibunda) skin secretions extract on wounds healing in mice. Iraqi J. Agricul. Sci., 49 (4): 316- 362.

Al-khateeb, S. O. 2016. Aqueous extract of red tea plants (Hibiscus sabdariffa L.) effect on the lipid profile and glugose in male white rabbits exposed to oxidative stress. Iraqi J. Agricul. Sci., 47(1):326-336

Al-Samurai, F. R. 2017. Biomedical Statistics book. Baghdad University, Iraq (in Arabic).

Bagheri, M., M. Fazli and S. Saeednia 2018. Pomegranate peel extract inhibits expression of beta-catenin, epithelial mesenchymal transition, and metastasis in triple negative breast cancer cells. Cell Mol. Biol., 64 (7): 86-91

Barku, V. Y., A. Boye and N. Quansah, .2013. Antioxidant and wound healing studies on the extracts of Corchorus olitorious leaf. World Essays J., 1 (3): 67-73

Barwary, M. S. Q., K. Y. Merkhan ,, E. T. S. Buti, R. H. Isa, K. N. Mustafa , and C. A. Yatem. 2019. Evaluation of medicinal plants (Astragalus eriocephalus and Quercus infectoria )as feeds additives in awassi ewes’ rations 2. Some blood biochemical, hormonal levels and milk hyigen. Iraqi J. Agricul. Sci., 50 (2): 526- 533

Chaitra, R. H., M. Madhur, T. Swaroop, D. Arijit, B. Sourav and K. C. Rohit. 2012. Evaluation of antimicrobial properties, phytochemical contents and antioxidant capacity of leaf extracts of Punica granatum L. J. Biol. Sci., 1: 32-37

Dan, T., L. Liu, A. Dildar, Y Jianping, X. Jianjun, X. Xuelei and A. Haji Akber. 2018. Anti-diabetic Effect of Punica granatum flower polyphenols extract in type 2 diabetic rats: Activation of Akt/GSK-3β and Inhibition of IRE1α-XBP1 pathways. Frontiers Endocrinol., 9: 1-11.

Debra, J. 2017 . How does my wound heal, and how do I treat it?. M. D., 10: 1-14.

Devansh, M. 2015. Wound healing medicinal plants: an exhaustive literature review. Inventi. Rapid. P. Acti., 1:1-11

Dhinesh, K. V. and D. Ramasamy 2016. Pomegranate(Punica granatum) processing and value addition: A review. J. Food Proc. Tech., 7 (3): 1-11

Farag, M. M. 2015. Isolation and Identifiration of Dermatophytes and Resistance of Some Extracts of Plants. M.Sc. Thesis. College of Science, University of Al. Mustansiriyah, Iraq.

Guo-Bing, S., W. Bing, W. Qiong, W. Tong-Chao, W. Chang-Li, S. Xue-Hui, Z. Wen-Tao, Y. Ming, Z. Qing-Chun, C. Yu-Feng and Z. Wei. 2014. Evaluation of wound healing activity and antiflammatory activity of aqueous extracts from Acorus calamus L. Pak. J. Pharm. Sci., 27 (1): 91-95.

Hardany, P., M. Y. Wiwik and S. L. Bambang. 2016. Benefits of pomegranate (Punica granatum Linn.) fruit extracts to weight changes, total protein, and uric acid in white rats (Rattus norvegicus) as an animal model of acute renal failure. Veterinary World, 9: 1269-1274.

Johan, B. E., A. K. Rami, E. J. Caterson and E. Eriksson. 2013. Clinical impact upon wound healing and inflammation in moist, wet, and dry environments. Adv. Wound Care, 2 (7): 348-356

Jumaa, F. F. and A. A. Ali. 2016. Effect of foliar application of potassium zinc and gibberelic acid on leaves and peel content of mineral elements of pomegranate. Iraqi J. Agricul. Sci., 47 (2) : 533-542

Kandemir, F. M., A. Sagliyan, M. Ozharaco, C. Gynay, M. C. Han and F. Benzer. 2013. Effects of oral administration of pomegranate seed extract on surgical wound healing in rabbits. Rev. Med. Vet., 164(8): 400-408

Karthikeyan, J., V. Kavitha, T. Abirami and G. Bavani. 2017. Medicinal plants and diabetes mellitus: A review. J. Pharm. Phytochem., 6 (4): 1270-1279

Kourl, I., S. Wakode, H. P. Singh and S. Manachanda. 2016. Development and validation of a stability indicating reverse phase HPLCPDA method for determination of canagliflozin in bulk and pharmaceutical dosage form. Pharm. Methods, 7(1):54-62

Kumarasamayraja, D., N. S. Jeganathan and R. Manavalan. 2012. A Review on medicinal plants with potential wound healing activity. Int. J. Pharm. Sci., 2 (4): 105-111.

Merkhan, K. Y., K. N. Mustafa, R. H. Isa, M. S. Q. Barwary, E. T. Buti and C. A. Yatem .2019. Evaluation of medicinal plants(and Quercus infectoria and Astragalus eriocephalus )as feeds additives in awassi ewe’s ration 1. digestibility, milk yield and composition . Iraqi J. Agricult. Sci., 50 (1): 525-115.

Mirele, S. V., F. G. Neuza, L. O. Maria, C. N. Diana, R. T. Adriana, Y. S. Francisco, G. P. Francisco, F. M. Carlos, R. M. Maria, F. M. Erika and F. M. Dirce. 2015. Anti-inflammatory and wound healing potential of cashew apple juice (Anacardium occidentale L.) in mice. Exp. Biol. Med., 240(12): 1648–1655

Park, S. Y., E. Y. Byun, J. D. Lee, S. Kim and H. S. Kim. 2018. Air pollution, autophagy, and skin aging: impact of particulate matter (PM10) on human dermal fibroblasts. Int. J. Mol. Sci., 19 (9): 1-27

Radenahmad, N., F. Saleh, I. Sayoh, K. Sawangjaroen, P. Subhadhirasakul, P. Boonyoung, W. Rundorn and W. Mitranun. 2012. Young coconut juice can accelerate the healing process of cutaneous wounds. BMC Complementary and Alternative Med., 12:252-258

Ronnie, G. 2019. Effect of pomegranate on cancer formation and growth. Life Extension Magazine, 30: 864-866

Satheesh, K. B., K. N. Suchetha, S. L. Vadisha, K. P. Sharmila and P. B. Mahesh. 2012. Preliminary phytochemical screening of various extracts of pomegranate (Punica granatum) peel, whole fruit and seeds. Nitte Univ. J. Health Sci., 2 (4): 34-38

Singh, D., B. Neena and A. K. Tiwary. 2019. Comparison of UV spectrophotometric and HPLC method for estimating canagliflozin in bulk and a tablet dosage form. Ind. J. Pharm. S., 8 (1): 39-44

Sreeja, S., S. Hima, M. Parvathy, M. A. Juberiya and S. Sreeja. 2014. Pomegranate fruit as rich source of biologically active compounds. Hindawi Pub. Corporation Bio. Med. Res. Int., 10: 6168- 6921

Visar, A., A. T. Pasha, J. F. Andrew, F. S. Michael, S. S. Daniel, S. Cristian, M. Y. Sulaiman, B. Dapeng, R. K. David, B. Shiladitya and P. M. Michael. 2019. Wound healing coordinates actin architectures to regulate mechanical work. Nat. Phys., 61: 1-16.

Walid, E., H. Hedia, T. Nizar, Y. Yassine, N. Nizar and F. Ali. 2012. Total phenolic contents and antioxidant activities of pomegranate peel, seed, leaf, and flower. J. Med. Plants Res., 6: 4724-4730.

Wesam, K., F. Maryam, A. Zahra, A. L. Damoon and A. S. Majid. 2016. The role of medicinal plants in the treatment of diabetes: a systematic review. Electron Physician, 8(1): 1832–1842.




How to Cite

Salim, F. D. ., Ibrahim, K. M. . and Yousif , W. H. . (2022) “THE EFFECTIVENESS OF EXTRACT THE SEED OF POMEGRANATE IN HEALING THE WOUND INDUCED INRABBITS SKIN ”, IRAQI JOURNAL OF AGRICULTURAL SCIENCES, 53(2), pp. 265–271. doi: 10.36103/ijas.v53i2.1533.