EVALUATION THE EFFECT OF GREEN SYNTHESIS TITANIUM DIOXIDE NANOPARTICLES ON ACINETOBACTER BAUMANNII ISOLATES

Abstract

Acinetobacter baumannii is gram negative opportunistic coccobacilli,the most important agent in nosocomial infections with high mortality rate. This bacteria is characterized by (biochemical tests, polymerase chain reaction (PCR) and vitek-2 system). The antibiotic susceptibility result of A. baumannii isolates were  shown resistant to (Methicillin, Nitrofurantion), while sensitive to (colistin, Ciprofloxacin).This study was aimed to biosynthesis of titanium dioxide nanoparticles by using prodigiosin pigment produced from clinical isolate Serratia marcescens as reducing and stabilizing agent for Titanium dioxide nanoparticles (TiO₂ NPs) and used as antibacterial agent for  Acinetobacter baumannii isolated from different clinical sources. TiO₂ NPs synthesized by using titanium chloride TiCl₄ (5 ml/50 ml) in deionized water with concentration of prodigiosin (10 mg/ml)and adjusted pH at 7 and the temperature at 50 ˚C. TiO₂NPs synthesized was Characterized by various technique, such as (AFM , UV-VIS, FTIR , XRD  and FE-SEM  ). The result showed that wavelength(366)nm  was optimum for TiO₂NPs that have crystalline shape  at average volume (47.52 nm). Green synthesized TiO₂ NPs have shown several applications such as biomedical, anticancer, bio sensing, catalysis etc.