IMPACT OF AFLATOXIN B1 ON GROWTH PERFORMANCE AND HISTOPATHOLOGICAL CHANGES IN CYPRINUS CARPIO
DOI:
https://doi.org/10.36103/4vnewz86Keywords:
carp, growth,kidney, muscles, mycotoxin, life below waterAbstract
This study aimed to identify the impact of aflatoxin B1 (AFB1)-contaminated diet on growth performance and histopathological changes of the kidney and muscle tissues in Cyprinus carpio . A total of 300 fish with an average of weight 45±5 g, were randomly distributed into 15 plastic tanks, and were divided into five experimental groups; (group 1) served as control , fish fed normal diet without solvent and AFB1, (group 2) positive control fish were fed with only solvent, and (groups 3-5) fish were fed diets containing 0.5, 1 and 2 mg AFB1/kg of feed, respectively. Results showed the negative effect for AFB1 on weight gain, average daily gain of fish. Histopathological changes of kidney and muscles in AFB1-treated fish showed devastation of the renal tract, glomerular atrophy with necrosis, expansion of Bowman’s space, necrosis in urinary tubules, peeling and degradation in the epithelium of tubules, vascular congestion, and interstitial hemorrhage associated with mononuclear cells (MNCs) infiltration with tubules, increased of urinary lumen space, and increase of melano-macrophage centers (MMC). The results also showed a significant damage to the structure of muscles as a result of the disintegration of muscle fibers, which increased with the increase in the AB1 concentrations.
References
Abdulrahman, N. M. , Z. S. Abdulla and S. M. Hassan. 2023. Histopathological changes of using raw lentil as a partial substitute for the fish meal in diets of the common carp Cyprinus carpio L. Iraqi Journal of Agricultural Sciences, 54(4):1137- 1146. https://doi.org/10.36103/ijas.v54i4.1807
Al-Aboudi H. J. , A. J. Al-Rudainy and A. A. Maktof. 2022 . Accumulation of lead and cadmium in tissues of Cyprinus carpio collected from cages of Al-Gharraf River / Thi Qar / Iraq. Iraqi Journal of Agricultural Sciences, 53(4):819-824. https://doi.org/10.36103/ijas.v53i4.1594
Anater, A., L. Manyesb, G. Mecab, E. Ferrerb., F. Bittencourt, C. Pimpãoa and G. Fontb. 2016. Mycotoxins and their consequences in aquaculture: A review. Aquaculture. 451: 1–10.
Applegate, T.J., G. Schatzmayer, K.Pricket, C. Troche and Z. Jiang. 2009. Effect of aflatoxin culture on intestinal function and nutrient loss in laying hens. Poultry Science. 88(6): 1235-1241.
Association of Official Analytical Chemist (AOAC). 2005. Official Methods Analysis. Natural Toxins, 17th ed. Chapter 49, Gaithersburg, MD.
Ayoola, S. 2011. Histopathology of Nile Tilapia (Oreochromis niloticus) juveniles exposed to aqueous and ethanolic extracts of Ipomoea aquatica leaf. Int. J. Fish and Aqua. Studies.3 (14):244-257.
El-Barbary, M. I. 2018. Impact of garlic and curcumin on the hepatic histology and cytochrome P450 gene expression of aflatoxicosis Oreochromis niloticus Using RT-PCR. Turk. J. Fish. Aquat. Sci. 18: 405-415.
Ghaednia, B., M. Bayat., I. Sohrabi, A. Motallebi and A. Sepahdari. 2013. Effects of aflatoxin B1 on growth performance, health indices, phagocytic activity and histopathological alteration in Fenneropenaeus indicus. Iran. J. Fish. Sci. 12(4):813-826.
He, C.,Y. Fan, Y. Wang, C. Huang, X. Wang and H. Zhang. 2010. The individual and combined effects of deoxynivalenol and aflatoxin B1 on primary hepatocytes of Cyprinus carpio. International Journal of Molecular Sciences.11: 3760-3768.
Huang, S.,J. Wang, H. Xing, X. Shen, J. Yan and X. Wang.2014. Impairment of cell cycle progression by sterigmatocystin in human pulmonary cells in vitro, Food and Chemical Toxicology. 66: 89-95.
Mustafa , S., A.J. Al-Rudainy and S.M. Al-Samawi. 2020. Histopathological and level of bioaccumulation of some heavy metals in fish Carabarbus luteus and Cyprinus carpio tissues caught from Tigris river, Baghdad. Iraqi Journal of Agricultural Sciences, 51(2):698-704. https://doi.org/10.36103/ijas.v51i2.997
Mustafa, S. and A. J. Al-Rudainy. 2021. Impact of mercury chloride exposure on some of immunological and biochemical assays of common carp, Cyprinus carpio. Iraqi Journal of Agricultural Sciences,52(3):547-551. https://doi.org/10.36103/ijas.v52i3.1341
Ramesh, F. and K. Nagarajan. 2013. Histopathological Changes in the Muscle Tissue of the Fish Clarias batrachus exposed to untreated and treated sago effluent. Advances in Bioscience and Bioengineering.1(2):74-80.
Shahafve, S., M. Banaee,N. Haghi and M. Mohiseni.2017. Histopathological study of common carp (Cyprinus carpio) fed aflatoxin-contaminated diets, Int. J. Aqua. Biol.5(2): 63-70.
Steinel, N. C. and D. I. Bolnick. 2017. Melanomacrophage centers as a Histological indicator of immune function in fish and other Poikilotherms. Frontiers in Immunology.8 (7):1-8.
Suvarna, K.S., C. Layton and J.D. Bancroft. 2012. Bancroft's Theory and Practice of Histological Techniques, 7th edition. E-Book. Elsevier Health Sciences.
Taheri, S., B. Mahdi,H. Behzad and M. Mohammad. 2017. Evaluation of nephrotoxic effects of aflatoxins on common carp (Cyprinus carpio). Iranian J. Toxicol. 11(2):51-58.
Yousefi, S., S. Dadgar , M. Safara and F. Zaini. 2016. Aflatoxin production by Aspergillus flavus isolates from green– tiger shrimps (Penaeus semisulcatus). Iranian J. Microbiol. 1(4): 18-2.
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