ACTIVE COMPOUNDS DETECTION IN AQUEOUS EXTRACT OF GANODERMA APPLANATUM LOCAL ISOLATE
DOI:
https://doi.org/10.36103/ijas.v54i5.1824Keywords:
GC-Mass Chromatography, phenols, flavonoids, tannins, FTIR.Abstract
This study was aimed to investigate active compounds in the aqueous extract of Ganoderma applanatum mycelium. The aqueous extract of fungus mycelium was prepared and some active compounds were detected, including phenols, flavonoids, tannins, alkaloids, glycosides and resins, all of which gave a positive detection. The effective compounds were diagnosed by GC-Mass Chromatography technique, was revealed that there are a number of compounds that can produce mycelium with ability to inhibit microorganisms, inhibit free radicals and prevent oxidation, as well as their effect in reducing cancerous tumors. As for the effective groups, they were diagnosed by FTIR technique.
References
Abdul Qadir, Z. M,AbdulIlahMekhlef Abdul Hadi, AdebaNajemRustam and Iyad Waleed Abdullah. 2014.Winning and reactivity for storage for oyster mushroom effect of different nitrogen sources. The Iraqi Journal of Agricultural Sciences. 45 (2):. 164-157
Al-Khesraji T. O., A.H.M. Shugran and R. Augul. 2017. Some basidioomycota macrofungal species from Salahdin Governorate (north central Iraq), with the addition of four new species to Iraq. International Journal of Current Research in Biosciences and Plant Biology,4(10):74-84. DOI: https://doi.org/10.20546/ijcrbp.2017.410.008
Ekhlas , M. F. and A.C, Rukaibaa .2019. Assessment of mutagenic and antimutagenic effects of honey formed by innovative way against cyclophosphamid induced chromosomal aberrations in bone marrow cells. Iraqi Journal of Agricultural Sciences 50 (5): 336-1344. https://doi.org/10.36103/ijas.v50i5.798
Farhan E. M. and R. A. Chechan.2017. Evaluting the ability of Pleurotus ostreatus aqueous extract to modulate genotoxicity induced by cyclophosphamide in mice bone marrow cells. Iraqi Journal of Agricultural Sciences,51(5):1405-1412.
Fu Y., L. Shi, and K. Ding.2019.Structure elucidation and anti-tumor activity in vivo of a polysaccharide from spores of Ganoderma lucidum (Fr.) Karst. International Journal of Biological Macromolecules, 141(1):693-699. DOI: 10.1016/j.ijbiomac.2019.09.046
Hyde K.D., C. J. Norphanphoun, A.J. Dissayake, M. Dollom, S. Hongsanan, S. Rurishikc, R.J. Jayawardena, H. P. Rekhani, T. Benjarong, N. Dhanvshka and D.N. wanasinghe.2018. Thailands amazing diversity: up to 96% of fungi in Northern Thailand may be novel. fungal diversity, 93(1): 215-239.
DOI: 10.1007/s13225-018-0415-7
Kozarski, M., A. Klaus, D. Jakovljevic, N. Todorovic, J. Vunduk, P. Petovic, M. Niksic, M. Vrvic and L. Griensven.2015. Antioxidants of edible mushrooms. Journal, Molecules, 20:19489-19525. doi: 10.3390/molecules201019489.
Li, Z., Y. Shi1, X. Zhang1, J. Xu2, H. Wang3, L. Zhao and Y. Wang.2020. Screening Immunoactive Compounds of Ganoderma lucidum Spores by Mass Spectrometry Molecular Networking Combined With in vivo Zebrafish Assays. Front. Pharmacol., 18 March. https://doi.org/10.3389/fphar.2020.00287
Lin, H.C., R.T. Hewage, Y.C. Lu and C. Yit-Heng.2019.Biosynthesis of bioactive natural products from basidiomycota. Journal Organic Biomolecular Chemistry, 17(5) 1027-1036. doi: 10.1039/c8ob02774a
Mohanta, Y.K., S.K. Singdevsachan, U.K. Umesh Kumar Parida, S.K. Sujogya Kumar Panda, T.K. Tapan Kumar Mohanta and H. Bae.2015.Green synthesis and antimicrobial activity of silver nanoparticles using wild medicinal mushroom Ganoderma applanatum (Pers.) Pat. from Similipal Biosphere Reserve, Odisha, India. IET Nanobiotechnol., pp. 1–6. doi: 10.1049/iet-nbt.2015.0059
Nagaraj, K., N. Mallikarjun , R. Naika and TM. Venugopal.2013. Phytochemical analysis and in vitro antimicrobial potential of Ganoderma applanatum (Pers.) Pat. of Shivamogga District-Karnataka, India. Int. J. Pharm. Sci. Rev. Res., 23(2),nᵒ 07, 36-41. Document 13309434
Orole, O.O.(2016).GC-MS Evaluation, phytochemical and antinutritional screening of Ganoderma lucidum. Journal of Advances in Biology & Biotechnology,5(4):1-10. DOI: 10.9734/JABB/2016/24261
Rukaibaa, A. C. ; O. M., Mohammed ; M., Ziena and M. M., Amar.2017.Prepration of new national media for cultivation and effect of some environmental factors on growth rate of oyster mushroom. Iraqi Journal of Agricultural Sciences 48(5),1304- 1312. https://doi.org/10.36103/ijas.v48i5.340
Sofowara A. 1993.Medicinal Plants and Traditional Medicine in Africa. Spectrum Books Ltd, Ibadan, Nigeria. pp 289
Sohretoglu D, S. Huang. Ganoderma lucidum polysaccharides as an anti-cancer agent.2018. Anticancer Agents Med Chem.,18(5):667-74.doi: 10.2174/187152061766 6171113121246
Sudheer S. , I. Alzorqi, S. Manickam and A. Ali.2019. Bioactive Compounds of the Wonder Medicinal Mushroom “Ganoderma lucidum”.Bioactive Molecules in Food, by Jean-Michel Mérillon and Kishan Gopal Ramawat, Springer Link. pp 1863-1893
Sulieman A. E., F. M. Issa and E. A. Elkhalifa.2007. Quantitative determination of tannin content in some sorghum cultivars and evaluation of its antimicrobial activity. Research Journal of Microbiology, 2: 284-288. DOI: 10.3923/jm.2007.284.288
Taşkın, H., E. Kafkas, Ö. Çakıroğlu and S. Büyükalaca.2013. Determination of volatile aroma compounds of Ganoderma lucidum by gas chromatography mass spectrometry (HS-GC/MS) Afr J Tradit Complement Altern Med.10(2):353-355. PMCID: PMC3746584.PMID: 24146461
Yao, Lu, Li-Ping Zhu, Xiao-Yan Xu, Ling-LingTan, Martin Sadilek, Huan Fan, Bo Hu, Xiao-Ting Shen, JieYang, BinQiao and SongYang.2016.Discovery of novel xylosides in co-culture of basidiomycetes Trametes versicolor and Ganoderma applanatum by Integrated Metabolomics and Bioinformatics. Open access, Nature, SR,6,33237.
https://www.nature.com/articles/srep33237
Zhu LF, Y. Yao, Z. Ahmad, MW. Chang. Development of Ganoderma lucidum spore powder based proteoglycan and its application in hyperglycemic, antitumor and antioxidant function. Process Biochemistry. 2019;84:103-11. DOI:10.1016/j.procbio.2019.05.025
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
2.jpg)
https://orcid.org/0000-0002-5774-5906 2.jpg)
