ANTIBACTERIAL PROPERTIES OF ZINC OXIDE NANOPARTICLES SYNTHESIZED BY THE SUPERNATANT OF WEISSELLA CONFUSA UPM22MT04
DOI:
https://doi.org/10.36103/ijas.v54i5.1816Keywords:
biosynthesis, Weissella confusa, ZnO-NPs, antibacterial activity, MRSA.Abstract
This study was aimed to produce zinc oxide nanoparticles (ZnO-NPs) using the supernatant of Weissella confusa UPM22MT04 and assess their effectiveness in inhibiting methicillin-resistant Staphylococcus aureus (MRSA). An isolate of Weissella confusa UPM22MT04 was isolated from a wastewater treatment plant in Johor, Malaysia, and was utilized to synthesize ZnO-NPs. The synthesized ZnO-NPs were characterized through several techniques, including UV-visible spectroscopy, Fourier-transform infrared spectroscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, and dynamic light scattering. Monodisperse spherical ZnO-NPs of 1.7 - 7.9 nm were obtained with 0.1 M zinc nitrate at 80°C. The biosynthesized ZnO-NPs exhibited vigorous inhibitory activity against MRSA. Results found that ZnO-NPs inhibited MRSA at a minimum concentration of 0.625 mg/mL and were bactericidal at a minimum concentration of 1.25 mg/mL. In MTT assays, ZnO-NPs showed no toxicity to HS-27 fibroblasts. The supernatant of Weissella confusa UPM22MT04 could be used to synthesize ZnO-NPs, which are an antibacterial agent, eco-friendly and non-toxic.
References
Abd Zaid, A. M., and N. J. Kandala. 2021. Identification of methicillin resistant Staphylococcus aureus using touchdown PCR and phenotypic methods from patients and hospitals environments in different Iraqi cities. Iraqi Journal of Agricultural Sciences, 52(6):1356-1364. https://doi.org/10.36103/ijas.v52i6.1475
Aiad, I., M.M. El-Sukkary, E.A. Soliman, M.Y. El-Awady, and S.M. Shaban, 2014. In situ and green synthesis of silver nanoparticles and their biological activity. Journal of Industrial and Engineering Chemistry, 20(5): 3430-3439.
Alden, M.A. and L.A. Yaaqoob, 2022. Evaluation of the Biological Effect Synthesized Zinc Oxide Nanoparticles on Pseudomonas aeruginosa. Iraqi Journal of Agricultural Sciences, 53(1): 27-37. https://doi.org/10.36103/ijas.v53i1.1502
Blanpain, C. and E. Fuchs, 2009. Epidermal homeostasis: a balancing act of stem cells in the skin. Nature reviews Molecular Cell Biology, 10(3): 207-217.
Brenner, D.J., N.R. Krieg, and J.T. eds. Staley, 2005. Bergey's Manual® of Systematic Bacteriology: Volume Two: The Proteobacteria (Part C). Springer US.
Chakansin, C., J. Yostaworakul, C. Warin, K. Kulthong and S. Boonrungsiman, 2022. Resazurin rapid screening for antibacterial activities of organic and inorganic nanoparticles: Potential, limitations and precautions. Analytical Biochemistry, 637:114449.
Djearamane, S., T.H. Ravintharan, S.X.T. Liang, and L.S. Wong, 2022. Sensitivity of Proteus vulgaris to Zinc Oxide Nanoparticles. Sains Malays, 51:1353-1362.
El-Sayed, H.S., S.M. El-Sayed and A.M. Youssef, 2021. Novel approach for biosynthesizing of zinc oxide nanoparticles using Lactobacillus gasseri and their influence on microbiological, chemical, sensory properties of integrated yogurt. Food Chemistry, 365: 130513.
El-Shanshoury, A.E., S.E. Elsilk and P.S. Ateya, 2013. Uptake of some heavy metals by metal resistant Enterobacter sp. isolate from Egypt. Afr. J. Microbiol. Res, 7(23): 2875-2884.
Fusco, V., G.M. Quero, G.S. Cho, J. Kabisch, D. Meske, H. Neve, W. Bockelmann and C.M. Franz, 2015. The genus Weissella: taxonomy, ecology and biotechnological potential. Frontiers in Microbiology, 6: 155.
Harandi, F.N., A.C. Khorasani, S.A. Shojaosadati and S. Hashemi-Najafabadi, 2021. Living Lactobacillus–ZnO nanoparticles hybrids as antimicrobial and antibiofilm coatings for wound dressing application. Materials Science and Engineering: C, 130: 112457.
Honary, S. and F. Zahir, 2013. Effect of zeta potential on the properties of nano-drug delivery systems-a review (Part 2). Tropical Journal of Pharmaceutical Research, 12(2): 265-273.
Jaddoa, N.T.M. and L.A. Gharb, 2021. The antibiofilm activity of Hibiscus sabdriffaL. against methicillin resistant staphylococcus aureus. Iraqi Journal of Agricultural Sciences. 52(3):626-631. https://doi.org/10.36103/ijas.v52i3.1352
Król, A., V. Railean-Plugaru, P. Pomastowski, M. Złoch and B. Buszewski, 2018. Mechanism study of intracellular zinc oxide nanocomposites formation. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 553: 349-358.
Kumar, S., G. Stecher, M. Li, C. Knyaz and K. Tamura, 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35(6): 1547.
Leite-Silva, V.R., W.Y. Sanchez, H. Studier, D.C. Liu, Y.H. Mohammed, A.M. Holmes, E.M. Ryan, I.N. Haridass, N.C. Chandrasekaran, W. Becker and J.E. Grice, 2016. Human skin penetration and local effects of topical nano zinc oxide after occlusion and barrier impairment. European Journal of Pharmaceutics and Biopharmaceutics, 104: 140-147.
Meyer, K., P. Rajanahalli, M. Ahamed, J.J. Rowe and Y. Hong, 2011. ZnO nanoparticles induce apoptosis in human dermal fibroblasts Via p53 and p38 pathways. Toxicology in Vitro, 25(8): 1721-1726.
Mohamed, A.A., M. Abu-Elghait, N.E. Ahmed and S.S. Salem, 2021. Eco-friendly mycogenic synthesis of ZnO and CuO nanoparticles for in vitro antibacterial, antibiofilm, and antifungal applications. Biological Trace Element Research, 199: 2788-2799.
Mohd Yusof, H., N.A. Abdul Rahman, R. Mohamad and U.H. Zaidan, 2020a. Microbial mediated synthesis of silver nanoparticles by Lactobacillus Plantarum TA4 and its antibacterial and antioxidant activity. Applied Sciences, 10(19): 6973.
Mohd Yusof, H., R. Mohamad, U.H. Zaidan and N.A. Abdul Rahman, 2019. Microbial synthesis of zinc oxide nanoparticles and their potential application as an antimicrobial agent and a feed supplement in animal industry: a review. Journal of Animal Science and Biotechnology, 10: 1-22.
Mohd Yusof, H., R. Mohamad, U.H. Zaidan and N.A.A. Rahman, 2020b. Sustainable microbial cell nanofactory for zinc oxide nanoparticles production by zinc-tolerant probiotic Lactobacillus plantarum strain TA4. Microbial Cell Factories, 19: 1-1.
Mohd Yusof, H., A. Rahman, R. Mohamad, U.H. Zaidan and A.A. Samsudin, 2020c. Biosynthesis of zinc oxide nanoparticles by cell-biomass and supernatant of Lactobacillus plantarum TA4 and its antibacterial and biocompatibility properties. Scientific Reports, 10(1): 1-13.
Moreno-Martin, G., M. Pescuma, T. Pérez-Corona, F. Mozzi and Y. Madrid, 2017. Determination of size and mass-and number-based concentration of biogenic SeNPs synthesized by lactic acid bacteria by using a multimethod approach. Analytica Chimica Acta, 992: 34-41.
Morowvat, M.H., K. Kazemi, M.A. Jaberi, A. Amini and A. Gholami, 2023. Biosynthesis and Antimicrobial Evaluation of Zinc Oxide Nanoparticles Using Chlorella vulgaris Biomass against Multidrug-Resistant Pathogens. Materials, 16(2): 842.
Mustafa, H.N. and I. Al-Ogaidi, 2023. Efficacy of zinc sulfide-chitosan nanoparticles against bacterial diabetic wound infection. Iraqi Journal of Agricultural Sciences,54(1):1-17. https://doi.org/10.36103/ijas.v54i1.1671
Nagajyothi, P.C., S.J. Cha, I.J. Yang, T.V.M. Sreekanth, K.J. Kim and H.M. Shin, 2015. Antioxidant and anti-inflammatory activities of zinc oxide nanoparticles synthesized using Polygala tenuifolia root extract. Journal of Photochemistry and Photobiology B: Biology, 146: 10-17.
Naskar, A. and K.S. Kim, 2019. Nanomaterials as delivery vehicles and components of new strategies to combat bacterial infections: Advantages and limitations. Microorganisms, 7(9): 356.
Nohynek, G.J., E.K. Dufour and M.S. Roberts, 2008. Nanotechnology, cosmetics and the skin: is there a health risk?. Skin Pharmacology and Physiology, 21(3): 136-149.
Obeizi, Z., H. Benbouzid, S. Ouchenane, D. Yılmaz, M. Culha and M. Bououdina, 2020. Biosynthesis of Zinc oxide nanoparticles from essential oil of Eucalyptus globulus with antimicrobial and anti-biofilm activities. Materials Today Communications, 25: 101553.
Ogunyemi, S.O., Y. Abdallah, M. Zhang, H. Fouad, X. Hong, E. Ibrahim, M.M.I. Masum, A. Hossain, J. Mo and B. Li, 2019. Green synthesis of zinc oxide nanoparticles using different plant extracts and their antibacterial activity against Xanthomonas oryzae pv. oryzae. Artificial cells, nanomedicine, and biotechnology, 47(1): 341-352.
Pomastowski, P., A. Król-Górniak, V. Railean-Plugaru and B. Buszewski, 2020. Zinc oxide nanocomposites—Extracellular synthesis, physicochemical characterization and antibacterial potential. Materials, 13(19): 4347.
Punjabi, K., S. Mehta, R. Chavan, V. Chitalia, D. Deogharkar and S. Deshpande, 2018. Efficiency of biosynthesized silver and zinc nanoparticles against multi-drug resistant pathogens. Frontiers in Microbiology, 9: 2207.
Rehman, S., B.R. Jermy, S. Akhtar, J.F. Borgio, S. Abdul Azeez, V. Ravinayagam, R. Al Jindan, Z.H. Alsalem, A. Buhameid and A. Gani, 2019. Isolation and characterization of a novel thermophile; Bacillus haynesii, applied for the green synthesis of ZnO nanoparticles. Artificial Cells, Nanomedicine, and Biotechnology, 47(1): 2072-2082.
Rasheed, H.T., K.J. Luti and M.A. Alaubydi, 2020. a probiotic application of Lactobacillus acidophilus ht1 for the treatment of some skin pathogens. Iraqi Journal of Agricultural Sciences, 51(6):1559–1571. https://doi.org/10.36103/ijas.v51i6.1183
Riaz, M., U. Sharafat, N. Zahid, M. Ismail, J. Park, B. Ahmad, N. Rashid, M. Fahim, M. Imran and A. Tabassum, 2022. Synthesis of biogenic silver nanocatalyst and their antibacterial and organic pollutants reduction ability. ACS Omega, 7(17): 14723-14734.
Selvarajan, E. and V.J.M.L. Mohanasrinivasan, 2013. Biosynthesis and characterization of ZnO nanoparticles using Lactobacillus plantarum VITES07. Materials Letters, 112: 180-182.
Singhal, G., R. Bhavesh, K. Kasariya, A.R. Sharma and R.P. Singh, 2011. Biosynthesis of silver nanoparticles using Ocimum sanctum (Tulsi) leaf extract and screening its antimicrobial activity. Journal of Nanoparticle Research, 13: 2981-2988.
Sujatha, J., S. Asokan and S. Rajeshkumar, 2021. Antidermatophytic activity of green synthesised zinc oxide nanoparticles using
Cassia alata leaves. Journal of Microbiology, Biotechnology and Food Sciences, 2021: 348-352.
Tettey, C.O. and Shin, H.M., 2019. Evaluation of the antioxidant and cytotoxic activities of zinc oxide nanoparticles synthesized using Scutellaria baicalensis root. Scientific African, 6: e00157.
Umavathi, S., M. Subash, K. Gopinath, S. Alarifi, M. Nicoletti and M. Govindarajan, 2021. Facile synthesis and characterization of ZnO nanoparticles using Abutilon indicum leaf extract: An eco-friendly nano-drug on human microbial pathogens. Journal of Drug Delivery Science and Technology, 66: 102917.
Zarei, M., A. Jamnejad and E. Khajehali, 2014. Antibacterial effect of silver nanoparticles against four foodborne pathogens. Jundishapur Journal of Microbiology, 7(1): e8720.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
2.jpg)
https://orcid.org/0000-0002-5774-5906 2.jpg)
