IN VITRO MICROGRAFTING OF RED GLOBE (VITIS VINIFERA L)
DOI:
https://doi.org/10.36103/ijas.v53i5.1621Keywords:
grape, micrografting, rootstocks, growth regulators, compatibility, cclimatizationAbstract
This study was carried out to evaluate the micrografting success of Red globe cultivar (Vitis vinifera L.) on three rootstocks Ramsey, Freedom and SO4 and investigate the effect of some factors on the micrografting success. Furthermore, compatibility in the graft union was studied. The results showed that Red globe on un-rooted Freedom achieved the highest percentages of scion survival (100.00 %), scion bud burst (60.00%), graft union formation (60.00%), and rooting (40.00%), also longest shoot (2.00cm), highest root number (8.00) and root length (4.50cm). Murashige and Skoog (MS) medium Supplemented with Indole Butyric Acid (IBA) at 1mgL-1 achieved the highest percentages of scion survival (95.20%), scion bud burst (65.43%), graft union formation (82.22%) also, longest shoots (1.84 cm) and maximum average leaf number (2.50). Regarding the effect of MS strength, 25% MS, 50% MS and 75% MS achieved positive effects on all parameters compared with full MS. The anatomical study revealed that Red globe on Freedom combinations were the best due to enough callus formation, less necrotic layers, and a wavy continuity of new cambium were determined. Sterilized 1:1:1 (v:v) peatmoss+perlite+sand mix gave the highest survival rate (100.00%) and showed superior vegetative growth. Freedom micrografts achieved the highest survival rate (100.00%), better growth and longer roots compared with those on SO4 and Ramsey (80, 70%) respectively.
References
Aazami, M.A and M.B.Hassanpouraghdam. 2010. In Vitro Micro-Grafting of Some Iranian Grapevine Cultivars. Romanian Biotech. Letters. 15, No.5
Aloni, B., R. Cohen., L. Karni., H. Aktas and M. Edelstein. 2010. Hormonal signaling in rootstock-scion interactions. Sci. Hortic.127, 119-126
Aydieh, A.A., M.K.H. Ibrahim and A. Ibrahim. 2000. In vitro propagation and fruiting of pineapple. Egypt. J. Hort. 27, 289-304.
Balta, F., R. Cangi., A. Dogan., T. Karadeniz and S.M Sen. 1996. Anatomical and histological investigations on graft union formation of iskenderiye misketi variety grafted on rupestris du lot rootstock. J. Agric. Sci. 6(2), 201–208
Baydar, N.G and H. Celik. 1999. The effects of shoot tip source on the success of in vitro micrografting in grapevine (Vitis vinifera L.). Turk. J Agri. 23, 741–748.
Bhatt, KM., F.A. Banday., M.A. Mir., Z.A. Rather and G. Hussain. 2013. In vitro grafting in apple (Malusdomestica. Borkh) cv. LalAmbri. Karnataka. Jour. Agric.Sci.26, 399-402
Bidarigh, S. and E. Azarpour. 2013. Study effect of BA, GA3 hormone and light rate of poinsettia under In Vitro condition. Inter. J. Agri. Crop Sci. 5(10), 1058-1063
Cakir, A., N. Karaca., M. Sidfar., C. Baral and G. Soylemezoglu. 2013. Determination of grafting success of sultani seedless grape variety on different American Rootstocks. J. Agric. Sci., 23(3), 229–235
Cangi, R., F. Balta and A. Dogan. 2000. Anatomical and histological investigations on the effects of stratification substrates on final take and quality of grafted vines. Turk. Agric. For. 24, 393–398
Castiglione, S., C. Franchin., T. Fossati., G. Lingua., P. Torrigiani and S. Biondi. 2007. High zinc concentrations reduce rooting capacity and alter metallothionein gene expression in white poplar (Populus alba L. cv. Villafranca). Chemosphere. 67 (11),17-26
Cohen, D. 1995. The culture medium. Acta Hort. 393, 15-24.
Dolgun, O., S.S. Ulas and T. Teker. 2016. Determination of Graft Success of Grape Cultivars Grafted on Two Different Roostocks. Acta Sci. Pol. Hortorum Cultus, 15(4) , 135-145
Dolgun, O., Tekintas, F.E., Ertan, E. 2008. An histological investigation of graft union in some plum varieties grafted on pixy rootstock. J. Adnan Menderes Univ. Agric. Fac. 5(1), 1–4
Duncan, D.B. 1955. Multiple range and multiple. F tests. Biometrics. 11: 1-42.
Dziedzic, E and M. Malodobry. 2006. Vegetative cherry rootstocks in tissue culture. Sodininkyste Ir Darzininkyste. 25, 77-84.
El-Hammady, M.A., F.A. El-Morshedy and N.A. Awad. 2012. Evaluation of Growth Parameters of both Dog Ridge, Salt Creek Rootstocks and Shoot Tip Micro-Grafting of Superoir and Thompson Seedless Cultivars Through in Vitro Culture. J. Plant Production, Mansoura Univ. 3 (8), 2347 – 2359
Estrada-Luna, A.A., C. López-Peralta and E. Cárdenas-Soriano. 2002. In vitro micrografting and the histology of graft union formation of selected species of prickly pear cactus (Opuntia spp.). Sci. Hortic.14, 317-327
Gambetta, G.A., T.L. Rost and M.A .Matthews.2009. Passive pathogen movement via open xylem conduits in grapevine graft unions. Am. J. Enol. Vitic. 60 (2), 241–245
Gargin, S and A. Altindisli. 2014. A research on the affinity coefficient of Red globe grape variety with 140R, 41B rootstocks. EDP. sci. 3, 1-5
Goyal, Y and H.C. Arya. 1981. Differentiation in cultures of Prosopis cineraria LinnCurr. Sci. 50, pp. 468-469
Hamad, A.M. 2014. Effect of peatmoss and sand mixing ratio on the acclimatization of Smooth cayenne pineapple (Ananas comosus (L) Merr.). Sci. Hum. St. Mag. Bangazy. 22, 1-10
Hamdan, A.J.S and S.B. Razq. 2010. Preliminary compatibility between some table-grapevine scion and phylloxera-resistant rootstocks cultivars. Jordan. J. Agric. Sci. 6(1), 1-10
Hartmann, H.T., D.E. Kester and J.R. Davies, 2002. Plant propagation: principles and practices. Prentice Hall, Edglewood Cliffs, NJ. 7th ed. P55.
Hassanen, S.A., A.I.A. Abido., M.A.M. Aly and G.A. Rayan. 2013. In vitro Preservationof Grapevine (Vitis vinifera L.) Muscat of Alexandria and Black Monukka Cultivars as Genetic Resource Middle-East Jour. Sci. Res. 13 (3), 328-337
Hassani, Z. 1990. Effect of β-indolylbutyric acid (IBA) on the performance of vine micrografts cultured in vitro. Progrès Agricole et Viticole. 107 (17) pp.375-379
Hossein, D.G., S. Farajollah and H. Hassanpour. 2008. Identification of graft incompatibility of pear cultivars on quince rootstock by using isozymes banding pattern and starch. Asian J. Plant Sci. 7, 109-112
Jamwal, M., B. Singh., N. Sharma., R. Kumar., A. Sharma., R.M. Sharma and A.M. Parmar. 2013. In vitro regeneration of grape (Vitis viniferaL.) cv. Per-lette. World. J. Agric. Sci. 9(2), 161-166.
Johanson, D.A. 1940. Plant Microtechnique. Mc Graw-Hill, Book Company, Inc New York and London.523pp
Kadleček, P., I. Tichá., V. Čapková and C. Schäfer. 1989. Acclimatization of micropropagated tobacco plantlets. In: Garab, G. (ed.): Photosynthesis: Mechanisms and Effects. Kluwer. Acad. Publishers. Dordrecht . V. Pp. 3853-3856
Khan, N., M. Ahmed, I. Hafiz, N. Abbas, S. Ejaz and M. Anjum. 2015. Optimizing the concentrations of plant growth regulators for in vitro shoot cultures, callus induction and shoot regeneration from calluses of grapes. J. Int. Sci. Vigne. Vin. 49, 37-45
Kilany, O.A., M.H. Abd El-Zaher and H.H. Hamed. 2012. The relationship between the histological features in the grafting areas and the compatibility degrees of some Mango cultivars onto Nucellar Seedlings. J. Hort. Sci & Ornamen. Plants. 4(1), 58-65.
Kim, C.S; C.H. Lee; H.S. Park and G.P. Lee. 2005. In vitro grafting of grape with Phylloxera resistant rootstock cultivars. Vitis 44, 195–196.
Kurten, U., A.M. Nautila, V. Kauppinen and M. Rousi. 1990. Somatic embryogenesis in cell cultures of birch (Betula pendula Roth.) Plant. Cell. Tiss. Org. Cult. 23, 101-105.
Leonardi, C and D. Romano. 2004. Recent issues on vegetable grafting. Acta. hort. (631), 163-174
Li, J.R. and G.W. Eaton. 1984. Growth and rooting of grape shoot apices in vitro. Hort.Sci.19, 64-66
Martínez, B.M.C., L.C. Alcaraz., B. Muries., C.C. Mota and M. Carvajal. 2010. Physiological aspects of rootstock-scion interactions. Sci. Hort. 127, 112-118
Mhatre, M and V.A. Bapat. 2007. Micrografting in grapevine (Vitis spp.) in: Protocols for Micropropagazion of Woody Trees and Fruits, Edired by Mohan Jain and H. Hăggman, Univ. of Finland, Ed. Springer, pp.249-259
Moore, R and B. Walker. 1981. Studies of vegetative compatibility–incomatibility in higher plants. II. A structural study of an incampatible heterograft between Sedum telephoides (Cras-sulaceae) and Solanum pennellii (Solanaceae). Amer. Jour. Bot. 68, 831–942
Moore, R. 1984. A model for graft compatibility-incompatibility in higher plants. Am. J. Bot. 71(5), 751-758
Murashige, T and F. Skoog, 1962. A revised medium for therapid growth and bioassay with tobacco tissue culture. Physiol. Plant. 15, 473–479.
Nhut, D.T., J.A.T. da Silva., P.X. Huyen and K.Y. Pack. 2004. The importance of explant source on regeneration and micropropagation of Gladiolus by liquid shake culture. Sci. Hort. Amsterdam. 102, p407-414
Olmstead, M., N.S. Lang., F.W. Ewers and S.A. Owens. 2006. Xylem vessel anatomy of sweet cherries grafted onto dwarfing and nondwarfing rootstocks. J. Am. Soc. Hortic. Sci. 131 (5), 577–585
Onay, A., V. Pirinc; H. Yildirim and D. Basaran. 2004. In vitro micrografting of mature pistachio (Pistacia vera var. Siirt). Plant Cell Tissue Organ Cult. 77, 215-219.
Patel, R.M and R.R. Shah. 2009. Regeneration of Stevia plant through callus culture. Indian .J. Pharm. Sci. 71(1), 46-50.
Polat, M., O. Dolgun., A. Yildirim., M.A. Askin and Z. Gokbayrak. 2010. Graft union formation of spur apple varieties grafted on different rootstocks. J. Food Agric. Environ., 8(2), 490–493
Pospíšilová, J., I. Tichá., P. Kadleček, D. Haisel and S. Plzáková. 1999. Acclimatization of micropropagated plants to ex vitro conditions. Biologia Plantar Um 42. (4), 481-497
Rehman, H.U and M.I.S. Gill. 2014. In vitro shoot tip grafting of Patharnakh [Pyruspyrifolia (Burm F.) Nakai] pear on Kainth rootstock. Vegetos 27, 363-369.
Rodrigues, A.C., C.A.P. Silveira., G.R. de L. Fortes., J.C. Fachinella and J.B. da Silva. 2003. Establishment and multiplication in vitro of Prunus sp. in different culture media. Rev. Bras. Frutic. 25, 131-133
Samaan, M.S.F. 2013. In vitro Propagation of Grape Transplants through Micrografting. Ph.D thesis. Fac. Agric. Ain Shams Univ. Egypt. Pp 265
Sander, J.F. 1993. Biochemisch physiologische Ursachen der durch die stickstoffernährug modiizierten Anfälligkeit des weizens (Triticum aestivum L). gegenűber dem Echten Mehitau (Erysiphe graminis DC f.sp. tritici Marchai) (PhD thesis). Culliververlag, Göttinen.148p (c.f.)
Sripaoraya, S.T., M. Robert., B. Power., J, Davey and R. Michael. 2003. Plant regeneration by somatic embyogenesis and organogenesis in commercial pineapple (Ananas comosus L) In vitro Cell. Devel. Biol. Plant. 39 (5), 450–454
Tangolar, S.G., K. Erçik and S. Tangolar. 2003. Obtaining Plants using in Vitro Micrografting Method in Some Grapevine Varieties (Vitis Vinifera L), Biotech & Biotech. Equipment. 17(2), 50-55
Tetsumura, T., Y. Matsumoto., M. Sato., C. Honsho., K. Yamashita., H. Komatsu., Y. Sugimoto and H. Kunitake. 2008. Evaluation of basal media for micropropagation of four highbush blue-berry cultivars. Sci. Hort. 119,72–74
Thimmappaiah, P., G.T. Puthra and S. Raichal. 2002. In vitro grafting of cashew (Anacardium occidentale L.). Sci. Hortic. 92,177-182
Usenik, V., k.B Krs., M. Vic and F.Sˇtampar. 2006. Early detection of graft incompatibility in apricot (Prunus armeniacaL.) using phenol analyses. Sci. Hortic.109: 332-338
Van Huylenbroeck, J.M and J. De Riek. 1995. Sugar and starch metabolism during ex vitro rooting and acclimatization of micropropagated Spathiphyllum "Petite" plantlets. Plant. Sci. 111: 19-25
Walker, M.A. and C.P. Meredith. 1990. The genetics of resistance to grapevine fanleaf virus (GFV) in Vitis vinifera. Vitis Special Issue, pp. 228–238.
Wang, G., X. Li., Q. Chen and J. Tian 2010. Studies on factors affecting the microshoot grafting survival of walnut. Acta Hort. 861:327-331.
Wang, Y and R. Kollmann. 1996. Vascular differantiation in the graft union of in vitro grafts with different compatibility. Structural and functional aspects. J. Plant Physiol., 147:521–533
Wilmoth, JC., S. Wang., S.B. Tiwari., A.D. Joshi., G. Hagen., T.J. Guilfoyle., J.M. Alonso., J.R. Ecker and J.W Reed. 2005. NPH4/ARF7 and ARF19 promote leaf expansion and auxin-induced lateral root formation. Plant J. 43: 118-130.
Yildirim, A.N., M. Polat., O. Dolgun., M.A. Askin., Z. Gokbayrak., B. San. 2010. Graft formation in some spur and vigorous apple varieties grafted on ottowa 3 rootstocks: A histological investigation. J. Food. Agric. Environ. 8(2), 512–514
Zhu, B., H.N. Cao., C.W. Zong., R.Z. Piao., L. Chen and L. Zhou. 2007. Micrografting Technology in Grapevine (Vitis vinifera L.). Fruit. Veget. Cereal Sci. Biotech. 1(1), 60-63.
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