ROLE OF DIMETHYL SULFATE ON BIOCHEMICAL CHARACTERISTICS OF Fragaria ananassa Duch UNDER SALINITY STRESS IN VITRO

Abstract

The aims of this study were to evaluate the effect of four concentrations of Dimethylsulfate (DMS) 0%, 0.03%, 0.06% and 0.1% under four levels of salinity including 0, 30, 60 and 90 mmol NaCl L^-1 on some biochemistry characters such as proline, hydrogen peroxide (H₂O₂), catalase (CAT), superoxide dismutase (SOD), chlorophyll a (Chl a), chlorophyll b (Chl b) and total chlorophyll. All treatments were distributed according to the factorial experiment using complete ro(CRD) with five replicates. Results revealed that 0.03% of DMS gave the highest proline of 2.08 mg g^-1, H₂O₂ of 0.64 µM 100mg^-1, catalase of 19.53 AU min^-1g^-1 and SOD of 116.8 IU 100mg^-1. The control achieved the highest Chl a  of35.75 mg g^-1, Chl b of 23.38 mg g^-1 and total chlorophyll of 79.00 mg g^-1 . Furthermore, 30 mM NaCl L^-1  possesses highest proline of 2.14 mg g^-1, H₂O₂ of 0.62  µM 100mg^-1, catalase of 19.38 AU min^-1g^-1  and SOD of 114.0IU 100mg^-1. The non-saline plantlets (control) gave highest Chl.a of 33.00 mg g^-1, Chl b 20.62 mg g^-1. While 60 mmol NaCl L^-1 gave biggest total chlorophyll of 74.75.