METABOLIC RESPONSES TO IN VITRO IN DROUGHT-TOLERANT IN A Cucurbita pepo L. ELICITED BY SALICYLIC ACID AND ZINC OXIDE NANOPARTICLES
DOI:
https://doi.org/10.36103/ijas.v54i5.1817Keywords:
antioxidant systems, secondary metabolites, sorbitol, plant growth regulators.Abstract
This experiment was conducted to investigate the effect of different zinc oxide nanoparticle (ZnONPs) concentrations and salicylic acid (SA) on growth characteristics and antioxidant defence from Cucurbita pepo L. callus culture in response to drought stress conditions. The study included two levels of drought induced by sorbitol (0 and 20 g l-1), two concentration of SA (0 and 20 mg l-1), and four concentrations of ZnONPs (0, 100, 200, and 300 ppm). Drought stress by sorbitol showed negative effects on some characteristics of callus culture fresh weight (FW), dry weight (DW), and hydrogen peroxide (H2O2). The exogenous SA showed a positive in most characteristics except for DW, H2O2 and CAT. ZnONPs positively affected all study indicators on tissue culture under drought conditions. The exogenous of both SA and ZnONPs without sorbitol increased significantly in FW and DW. The SA and ZnO NPs with sorbitol increased significantly in biochemical characteristics such as H2O2, SOD, CAT, proline, and phenolic compounds such as coumaric acid, ferulic acid, caffeic acid, luteolin, and rutin.
References
Abdelnasser, G. 2012. Improving effect of salicylic acid on the multipurpose tree Ziziphus spina-christi L. willd tissue culture. American Journal of Plant Sciences, 3(7): 947-952
Aebi, H. 1984. Catalase in vitro. Methods in Enzymology, 105: 121–126.
Ahmed, T. S. and A. Y. Salih. 2023. Effect of foliar application of salicylic acid, magnesium, and iron on the seedligs of Citrus medica L., 54(2):388-398. https://doi.org/10.36103/ijas.v54i2.1713
AL-Jeboori, K. D., M. Z. K. AL-Mharib, A. Q. Hamdan, and A. H. Mahmood. 2017. Effect of irrigation intervals and foliar of salicylic acid on growth and yield of Potato. Iraqi Journal of Agricultural Sciences, 48(1): 242–247. https://doi.org/10.36103/ijas.v48i1.440
Al-Khafaji, A. M. H. H., and K. D. H. Al-Jubouri. 2023. Upgrading growth, yield, and folate levels of lettuce via salicylic acid and spirulina, vermicompost aqueous extracts, 54(1):235-241. https://doi.org/10.36103/ijas.v54i1.1696
Azzedine, F., H. Gherroucha, and M. Baka, 2011. Improvement of salt tolerance in durum wheat by ascorbic acid application. Journal of Stress Physiology and Biochemistry, 7(1): 27–37
Bates, L. S., R.R. Waldren, and I.D. Teare, 1973. Rapid determination of free proline for water-stress studies. Plant and Soil, 39: 205–207
Beyer Jr, W. F., and I. Fridovich, 1987. Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Analytical Biochemistry, 161(2): 559–566
Chahardoli, A., N. Karimi, F. Sadeghi, and A. Fattahi, 2017. Green approach for synthesising gold nanoparticles from Nigella arvensis leaf extract and evaluating their antibacterial, antioxidant, cytotoxicity, and catalytic activities. Artificial Cells. Nanomedicine, and Biotechnology. 46(3): 579-588.
Chung, I., M. Thiruvengadam, K. Rekha, and G. Rajakumar, 2016. Elicitation enhanced the production of phenolic compounds and biological activities in hairy root cultures of bitter melon Momordica charantia L. Brazilian Archives of Biology and Technology. 59: 1–10
Dar, A.H., S.A. Sofi, and S. Rafiq, 2017. Pumpkin the functional and therapeutic ingredient: A review. International Journal of Food Sciences and Nutrition. 2(6): 165–170
Demiralay, M., A. Saglam, and A. Kadioglu, 2013. Salicylic acid delays leaf rolling by inducing antioxidant enzymes and modulating osmoprotectant content in Ctenanthe setosa under osmotic stress. Turkish Journal of Biology. 37(1): 49–59.
Elbasheer, Y.H.A., S.A. Alem, and E.I.E. Osman, 2019. Feasible surface sterilization protocol for microbial contamination of some forest trees on in vitro cultures explants. Acta Scientific Microbiology. 2(5): 55–60
Foyer, C. H., and J.M. Fletcher, 2001. Plant antioxidants: colour me healthy. Biologist. 48(3): 115–120
Gutierrez, R.M.P. 2016. Review of Cucurbita pepo (pumpkin) its phytochemistry and pharmacology. Medicinal Chemistry. 6: 12–21
Hernandez-Viezcas, J.A., H. Castillo-Michel, A.D. Servin, J.R. Peralta-Videa, and J.L. Gardea- Torresdey, 2011. Spectroscopic verification of zinc absorption and distribution in the desert plant Prosopis juliflora-velutina (Velvet mesquite) treated with ZnO nanoparticles. Chemical Engineering Journal. 170(1–3): 346–352
Horvath, E., G. Szalai, and T. Janda, 2007. Induction of abiotic stress tolerance by salicylic acid signaling. Journal of Plant Growth Regulation. 26: 290–300
Iziy, E., A. Majd, M.R. Vaezi-Kakhki, T. Nejadsattari, and S.A. Noureini, 2019. Effects of zinc oxide nanoparticles on enzymatic and non-enzymatic antioxidant content, germination, and biochemical and ultrastructural cell characteristics of Portulaca oleracea L. Acta Societatis Botanicorum Poloniae. 88(4): 1–14.
Kulczynski, B., and A. Gramza-Michałowska, 2019. The profile of secondary metabolites and other bioactive compounds in Cucurbita pepo L. and Cucurbita moschata pumpkin cultivars. Molecules. 24(16): 2945-2967
Kwon, Y. I., E. Apostolidis, Y.C. Kim, and K. Shetty, 2007. Health benefits of traditional corn, beans, and pumpkin: in vitro studies for hyperglycemia and hypertension management. Journal of Medicinal Food. 10(2): 266–275
Li, X., Y. Yang, L. Jia, H. Chen, and X. Wei, 2013. Zinc-induced oxidative damage, antioxidant enzyme response and proline metabolism in roots and leaves of wheat plants. Ecotoxicology Environmental Safety. 89: 150–157
Mradu, G., S. Saumyakanti, M. Sohini, and M. Arup, 2012. HPLC profiles of standard phenolic compounds present in medicinal plants. International Journal of Pharmacognosy and Phytochemical Research. 4(3): 162–167
Mohammed, A. A., J. M. Abbas, and M. H. K. Al-Baldawi. 2020. Effect of salicylic acid spraying on yield and it's components of linseed cultivars. Iraqi Journal of Agricultural Sciences, 51(2):585-591. https://doi.org/10.36103/ijas.v51i2.985
Murashige, T, and F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco cultures. Physiologia Plantarum. 15: 473-497
Naderi, M.R., and A. Danesh-Shahraki, 2013. Nanofertilizers and their roles in sustainable agriculture. International Journal of Agriculture and Crop Sciences. 5 (19): 2229–2232
Neamah, S. I., and A. F. Almehemdi, 2017. Extraction of natural compounds from callus inducted of common sage plant Salvia officinalis L. and their evalution of antioxidant activity. Iraqi Journal of Agricultural Sciences. 48(6): 1541–1548
Neamah, S. I. 2018. In vitro production of some terpenoids compounds from Nigella sativa with different explants type and PEG concentrations. Iraqi Journal of Agricultural Sciences. 49(4): 534–540. https://doi.org/10.36103/ijas.v49i4.60
Othman, M., M. M. Al-Zoubi, and A. Sh. Al-Ouda, 2020. Role of conservation agriculture in improving production water and nitrogen efficiency of wheat under rainfed conditions. Iraqi Journal of Agricultural Sciences, 51(4): 1139–1148. https://doi.org/10.36103/ijas.v51i4.1093
Parveen, A., and S. Rao, 2015. Effect of nanosilver on seed germination and seedling growth in Pennisetum glaucum. Journal of Cluster Science. 26 (3): 693–701
Rad, Z. M. H. Nourafcan1, N. Mohebalipour,A. Assadi, and S. Jamshidi. 2021. Effect of salicylic acid foliar application on phytochemical composition, antioxidant and antimicrobial activity of silybum marianum. Iraqi Journal of Agricultural Sciences,52(1):63-69. https://doi.org/10.36103/ijas.v52i1.1236
Ramakrishna, A., and G.A. Ravishankar, 2011. Influence of abiotic stress signals on secondary metabolites in plants. Plant Signaling and Behavior. 6(11):1720–1731.
Rao, S., and G.S. Shekhawat, 2014. Toxicity of ZnO engineered nanoparticles and evaluation of their effect on growth, metabolism and tissue specific accumulation in Brassica juncea. Journal of Environmental Chemical Engineering. 2(1): 105–114
Regni, L., D. D. Buono, M. Micheli, S. L. Facchin, C. Tolisano, and P. Proietti, 2022. Effects of biogenic ZnO nanoparticles on growth, physiological, biochemical traits and antioxidants on olive tree in vitro. Horticulturae. 8 (161):1-13
Salih, A.M., F. Al Qurainy, S. Khan, M. Tarroum, M. Nadeem, H.O. Shaikhaldein, A. ZakariaGaafar, and N. S.Alfarraj, 2012. Biosynthesis of zinc oxide nanoparticles using phoenix dactylifera and their effect on biomass and phytochemical compounds in Juniperus procera. Scientifc Reports, 11: 1-12.
Sami, F., M. Yusuf, M. Faizan, A. Faraz, and S. Hayat, 2016. Role of sugars under abiotic stress. Plant Physiology and Biochemistry. 109: 54–61
Sarmadi, M., N. Karimi, J. Palazon, A. Ghassempour, and M.H. Mirjalili, 2018. The effects of salicylic acid and glucose on biochemical traits and taxane production in a Taxus baccata callus culture. Plant Physiology and Biochemistry. 132: 271–280.
Saruhan, N., A. Saglam, and A. Kadioglu, 2012. Salicylic acid pretreatment induces drought tolerance and delays leaf rolling by inducing antioxidant systems in maize genotypes. Acta Physiologiae Plantarum. 34: 97–106
Sen, A. 2012. Oxidative Stress Studies in Plant Tissue Culture. Antioxidant Enzyme, p 30
Sharma, A., G.P.S. Sidhu, F. Araniti, A.S. Bali, B. Shahzad, D.K. Tripathi, M. Brestic, M. Skalicky, and M. Landi, 2020. The role of salicylic acid in plants exposed to heavy metals. Molecules. 25(3): 1–21.
Sheykh, B.N., G.T.A.A. Hasanzadeh, M.M.A. Baghestani, and B. Zand, 2009. Study the effect of zinc foliar application on the quantitative and qualitative yield of grain corn under water stress. Electronic Journal of Crop Production. 2 (2): 59-73
Tripathi, D.K., V.P. Singh, S.M. Prasad, D.K. Chauhan, N.K. Dubey, and A.K. Rai, 2015. Silicon-mediated alleviation of Cr (VI) toxicity in wheat seedlings as evidenced by chlorophyll florescence, laser induced breakdown spectroscopy and anatomical changes. Ecotoxicology Environmental Safety. 113: 133–144
Turgut, R., and A. Kadioglu, 1998. The effect of drought, temperature and irradiation on leaf rolling in Ctenanthe setosa. Biologia Plantarum. 41: 629–633
Wang, J., J. Qian, L. Yao, and Y. Lu, 2015. Enhanced production of flavonoids by methyl jasmonate elicitation in cell suspension culture of Hypericum perforatum. Bioresources and Bioprocessing. 2(5): 1-9.
Yasir, S.S., R. M. Hamad and S. I. Neamah. 2022. Role of dimethyl sulfate on biochemical characteristics of Fragaria ananassa Duch under salinity stress in vitro, Iraqi Journal of Agricultural Sciences, 53(1):111-121. https://doi.org/10.36103/ijas.v53i1.1514
Yordanova, R., and L. Popova, 2007. Effect of exogenous treatment with salicylic acid on photosynthetic activity and antioxidant capacity of chilled wheat plants. General and Applied Plant Physiology. 33(3–4): 155–170
Zoufan, P., M. Baroonian, and B. Zargar, 2020. ZnO nanoparticles-induced oxidative stress in Chenopodium murale L. Zn uptake, and accumulation under hydroponic culture. Environmental Science and Pollution Research, 27: 11066–11078.
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