DETECTION OF THE MOST IMPORTANT PATHOGENIC BACTERIA AFFECT EXTERNAL ORGANS OF CYPRINUS CARPIO IN WASIT PROVINCE
DOI:
https://doi.org/10.36103/ijas.v53i5.1624Keywords:
fish- carp- skin lesion- Aeromonas hydrophila - Pseudomonas aeruginosa - Citrobacter freundiiAbstract
The present study was aimed to find the most common causative agents relates to skin ulceration in most common fish farming (ponds and cages) affects Cyprinus carpio in Wasit province harvested from three different regions. The laboratory isolation and detection of bacteria in this study was carried out in medical laboratory techniques in Middle Technical University and Central Public Health Laboratory in Baghdad from September to December 2020. For this study 240 infected common carp Cyprinus carpio L. were used, 120 samples from cages and 120 samples from ponds. Samples were taken from three regions, which represented the north, central, and south of the governorate. Samples from skin ulcers were taken from affected areas in the body of the fish, skin, gills and fins, then transporting to the laboratory to perform bacterial culture. The present study illustrates that the common causative agent of ulceration in fish skin, gills and fins was bacteria with most detected bacteria were Aeromonas hydrophila (56.7%) in cages and (37.5%) in ponds, followed by Pseudomonas aeruginosa (40.83%) in cages and (35.08%) in ponds, and Citrobacter freundii (41.66%) in cages and (25.8%) in ponds through the months including in this study. In conclusion, the most important pathogenic bacteria related to skin ulceration in both cages and ponds in all three locations in Wasit province were Aeromonas hydrophila, Pseudomonas aeruginosa and Citrobacter freundii. The infection rate was higher in cages compared to ponds because of increase pollution by sewage and organic compound and due to stress condition resulting from higher stoking density.
References
Abid, O.I. and A.H. Al-Hamdani. 2016. Study of the causative agents of ulcerated skin lesions of carp fish ponds at Sulaimani province. Basrah Journal of Veterinary Research. 15(3): 1-19
Al-Hamed, M. I. 1960. Carp-culture in Iraq. Iraqi Journal of Agricultural Research. 1 (2): 14-23
Al-Rudainy, A.J. and A.H.K., Salman, 2013. Determination Optimal Level of Ozone in the Treatment of Disease of Common Carp Cyprinus carpio in Water Contaminated with the Bacteria Aeromonas hydrophila. Basrah Jour
Annas, S., M. Zamri-Saad, F.F.A. Jesse and Z. Zunita. 2014. New sites of localisation of Pasteurella multocida B:2 in buffalo surviving experimental haemorrhagicsepticaemia. BMC Vet. Res. 10 (88).
Anshary, H., R.A. Kurniawan, S. Sriwulan R., Ramli, and D.V. Baxa. 2014. Isolation andmolecular identification of the etiological agents of streptococcosis in Nile Tilapia (Oreochromis niloticus) cultured in net cages in Lake Sentani, 3.SpringerPlus, Papua, Indonesia. 627
Austin, B. and D. A. Austin. 2016. Bacterial Fish Pathogens: Disease of Farmed and Wild Fish. Edn 6, Springer, New York. pp : 732.
Falagas, M.E., P.K. Kavvadia, E. Mantadakis, D.P. Kofteridis, I.A. Bliziotis, E. Saloustros, S. Maraki and G. Samonis. 2006. Morganella morganii infections in ageneral tertiary hospital. Infection. 34 (6): 315–321
Idowu, A. A., N. B. Ikenweiwe, and A. A. Alimi. 2013. Effects of some synthetic antibiotics on Streptococcus pneumoniae and Proteus mirabilis isolated from fish tank culture system. International Journal of Agriculture, Forestry and Fisheries. 1 (1): 1-5
Jassim, A.A.R., Abdulhameed, D.B. and Al Shammari, N.R., 2019. Bacterial Fish Diseases in some Semi-close Aquaculture Systems in Basrah Province, Iraq. Basrah J. Agric. Sci., 32, pp.75-84
Jawetz, E., J. L. Melnick, E. A. Adelberg, G. F. Brooks, J. S. Butel and L. N. Ornston. 2005. Mikrobiologi kedokteran. Jakarta: EGC
Khamees, E.S., A. J. Al-Rudainy, and E. B., Faleh, 2013. Study of Histopathological Changes in the Common Carp (Cyprinus carpio) experimentally infected by bacteria aeromonas hydrophila. Basrah Journal of Agricultural Sciences, pp:26.
Alsaphar, S.A., 2012. Detection and study of the experimental infection of Aeromonas strain in the common carp (Cyprinus carpio L.). Iraqi Journal of Veterinary Medicine, 36(2), pp.222-230
Law, M. 2001. Differential diagnosis of ulcerative lesions in fish. Environmental Health Perspectives. 109 (5): 681–686
Lee, S., M. Najiah, W. Wendy and M. Nadirah. 2010. Antibiogram and heavy metal resistance of pathogenic bacteria isolated from moribund cage cultured silver catfish (Pangasius sutchi) and red hybrid tilapia (Tilapia sp.). Front. Agric. 4. (1): 116–120
Lee, S.W., M. Najiah, T.S. Chuah, A.M.S. Noor, W. Wendy, M. Nadirah and M.A.W. Effendy. 2011. Antibiogram and plasmid profiling from Edwardsiella tardaisolated from freshwater fish in east coast Malaysia. J. Sust. Sci. Manage. 6 (1),19–27
Marcel, G., M.Y. Sabri, A. Siti-Zahrah and B.O. Emikpe. 2013. Water condition andidentification of potential pathogenic bacteria from red tilapia reared in cagecultured system in two different water bodies in Malaysia. Afr. J. Microbiol.Res. 7 (47): 5330–5337
McConnell, M. J., L. Actis and J. Pachón. 2013. Acinetobacter baumannii humaninfections, factors contributing to pathogenesis and animal models. FEMSMicrobiol. Rev. 37 (2): 130–155
Mermin, J., L. Hutwagner, D. Vugia, S. Shallow, P. Daily, J. Bender, J. Koehler, R. Marcus and F.J. Angulo. 2004. Reptiles, amphibians, and human Salmonellainfection: a population-based, case-control study. Clin. Infect. Dis. 38 (3): 253–261
Mhaisen, F. T. 1993. A review on the parasites and disease in fish ponds and farms of Iraq. Iraqi Journal of Veterinary Science. 6 (2): 20-28
Netto, L. N., C. A. G. Leal and H.C. P. Figueiredo. 2011. Streptococcus dysgalactiae as anagent of septicaemia in Nile tilapia, Oreochromis niloticus (L.). J. Fish Dis. 34 (3): 251–254
Noga, E. J. 2010. Fish Disease: Diagnosis and Treatment (Second edition). Wiley Blackwell Publication, USA
Pennell, W. and B. A. Barton eds., 1996. Principles of salmonid culture. Elsevier
Procop, G. W., D. L. Church, G. S. Hall, and W. M. Janda. 2017. 7th ed. Koneman's Color Atlas and Textbook of Diagnostic. Philadelphia: Wolters Kluwer. Pp: 845-853
Roberts, H. E. 2010. External Gram-Positive Bacterial Infection: Fundamentals of Ornamental Fish Health. First edition. Blackwell Publishing. London
Shayo, S. D., C. J. Mwita, and K. M., Hosea. 2012. Virulence of Pseudomonas ndAeromonas bacteria recovered from Oreochromis niloticus (Perege) from Mterahydropower Dam. Tanzania. Ann. Biol. Res. 3 (11): 5157–5161.
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