RAPID PROTOCOL OF Aloe vera In Vitro propagation

Abstract

The current research was carried out to enhance Aloe vera propagation via tissue culture technique. BA at 0, 1, 2, 3, and 4 mg/l alone or combined with 0.1 mg/l NAA were used for shoot proliferation. The best result was recorded by using 2mgl-1 BAP (5.33 shoots/ explant). Meanwhile, combination between 2 mg/l BAP with (0.2, 0.4 and 0.6) mg/l kinetin, NAA or IBA were tested and best protocol was shown by using 2 mg1-1 BAP + 0.6 NAA which recorded 4.89 shoots/ explant. For rooting, MS medium at half and full strength salts were used supplemented with 0, 1, 2, 3, 4mgl-1 of NAA. The result revealed that 3 mg1-1 NAA at half strength of MS medium regenerate developed roots (8.67 roots / shoot) within 4 weeks. The well successful healthy plantlets were transferred into a potting mix composed of sand and peat moss which shows 100% survival ratio.