TRADITIONAL AND MOLECULAR STUDY OF CRYPTOSOPORIDIUM SPP. IN DOMESTIC DOGS IN BAGHDAD CITY,IRAQ
The aim of this study to investigate the prevalence of Cryptosoridium spp. in domestic dogs in Baghdad city, by using microscopic examination(flotation and staining) and molecular techniques .The results revealed that the rate of Cryptosporidium infection was 15% by microscopic examination and 28.6% using PCR, our study showed that the infection rate was 8.5% in males and 21.42% in females by microscopic examination , while the infection rate by using PCR in males and females was 21.42%, 35.71% respectively. The percentage of infection in the age groups <1 year and >1 year was 20%, 10% respectively by microscopic examination, while by using molecular assay the rate of infection in the age groups <1 year was 40% and 17.14% in >1 year. This is the first study in Iraq to detect Cryptosoridium spp. in domestic dogs using molecular technique.